Marking method for indicating and distinguishing domestic and wild Bactrian camel DNA microsatellites

Abstract

The invention relates to a marking method for indicating and distinguishing domestic and wild Bactrian camel DNA microsatellites in the technical field of bio-engineering. The steps of the method are as follows: ear tissue samples of Bactrian camels are collected, and genomic DNA is extracted according to the conventional phenol / chloroform method; PCR amplification is carried out on the genomic DNA of the Bactrian camels by means of using 16 pairs of designed primers; PCR products are separated by means of using non-denatured polyacrylamide gel electrophoresis, and genotype is classified according to the lengths of the PCR products; distribution of different microsatellite-marked genotypes in the Bactrian camels is compared, and the result shows that allele-specific genes of 3 microsatellite loci (VOLP-10, YWLL36 and YWLL38) exist between domestic and wild Bactrian camels; allele-specific amplified products are cloned and sequenced. The method can be operated simply and can distinguish the domestic Bactrian camels and the wild Bactrian camels at the genome molecular level, thereby providing an important means and method for researching the heredity and the evolution of the Bactrian camels, especially the identification and the protection of the wild Bactrian camels.

Description

technical field [0001] The invention relates to a marking method in the technical field of bioengineering, in particular to a marking method for predicting and distinguishing DNA microsatellites of domesticated and wild Bactrian camels. Background technique [0002] Microsatellites are called Simple Sequence Repeats (SSRs) or short tandem repeats (STRs) or short tandem repeat polymorphism (Short Tandem Repeat Polymorphism, STRP) or simple sequence length polymorphism (Simple Sequence Length Polymorphism, SSLP ), etc., refers to multiple tandem repeated DNA sequences (mostly 1 to 6 nucleotides) with a few nucleotides as the unit, and its length is generally about 200bp (Beckmann et al., "Gennomics", 1992, 12 vol, pp. 281–288). Microsatellite DNA tandem repeats are distributed in the whole genome of livestock and poultry, and the number of repeats ranges from 10 to 60 times. The loci are composed of microsatellites and flanking sequences on both sides. Microsatellite markers...

AI Technical Summary

Problems solved by technology

At present, there are few studies on camel DNA microsatellites, and the number of camel microsatellites obtained is also very limited. Therefore, the development and utilization of microsatellite markers is a very important technical problem for solving the research work of Bactrian camel molecular genetics and molecular evolution. Urgent task

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