Jiaxing black pig TFB2M gene characteristic sequence, primer and detection kit

A technology for detecting kits and genetic characteristics, which is applied in genetic engineering, plant genetic improvement, microbial measurement/inspection, etc., and can solve problems such as low feed conversion rate, low lean meat rate, and shrinking population size

Active Publication Date: 2018-10-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the slow growth rate, low slaughter rate, low slaughter rate, low lean meat rate, and low feed conversion rate of local pig breeds in my country, they cannot adapt to the industrial development of the pig industry, nor can they meet the growing demand for lean pork in the market. demand, resulting in a shrinking group size (Liu Yingying et al., 2015)
The practice of pig breeding in the 20th century showed that excessive growth rate and excessive lean meat rate lead to the decline of pork quality and the reduction of individual viability
But so far, the reports on TFB2M and TFB2M genes related to quality traits of Jiaxing black pork are still blank

Method used

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  • Jiaxing black pig TFB2M gene characteristic sequence, primer and detection kit
  • Jiaxing black pig TFB2M gene characteristic sequence, primer and detection kit
  • Jiaxing black pig TFB2M gene characteristic sequence, primer and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Materials and methods

[0034] 1.1.1 Experimental materials

[0035] 1.1.1.1 Experimental animals

[0036] A healthy Jiaxing black pig of about 72kg was selected, provided by Zhejiang Qinglian Food Co., Ltd. After slaughtering, the livers were taken out, placed in cryopreservation tubes and put into liquid nitrogen tanks for quick freezing. After returning to the laboratory, they were taken out of the liquid nitrogen tanks and stored in a -80°C refrigerator for later use as test samples.

[0037] 1.1.1.2 Main instruments and reagents

[0038] 1.1.1.2.1 Main instruments

[0039]

[0040] 1.1.1.2.2 Main reagents

[0041] Trizol A + Reagents, plasmid small extraction kit (No.DP103), DH5α competent cells (No.CB101), DL2000 Marker DL1000 Marker were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; Prime Script RTreagent Kit with gDNA Eraser (No.DRR037A ), EX Taq (No.DR001A), and Gel Extraction Kit (gel recovery kit) were purchased from Treasure...

Embodiment 2

[0150] Example 2: Analysis of differential expression of TFB2M gene in different tissues of Jiaxing black pig

[0151] 2.1 Materials and methods

[0152] 2.1.1 Sample material

[0153] 2.1.1.1 Test sample

[0154]Choose 10 Jiaxing black pigs that are healthy and reach the market slaughter weight, 5 Berkshire x Jiaxing black pigs, 5 Landrace x Jiaxing black pigs, 5 Du x Bajia pigs, 5 Du x Changjia pigs, and 5 Berkshire pigs. The scissors and tweezers used for tissue collection were sterilized at high temperature before being used for later use. After slaughtering, 8 tissues including heart, liver, spleen, lung, sebum, abdominal fat, stomach, and leg muscles were collected quickly, put them into cryopreservation tubes, quickly put them into a liquid nitrogen tank, and bring them back to the laboratory. Store in -80°C refrigerator for later use.

[0155] 2.1.1.2 Test equipment

[0156] Scissors, tweezers, freezing tubes, liquid nitrogen, freezing boxes, real-time fluorescent...

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PUM

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Abstract

The invention relates to a Jiaxing black pig TFB2M gene characteristic sequence and a specific amplification primer, and a detection kit for Jiaxing black pig pork quality related traits including theTFB2M gene characteristic sequence and the specific amplification primer. Correlation analysis between the expression level of the TFB2M gene and the pork quality traits and carcass traits of Jiaxingblack pigs is carried out, and a genetic mechanism related to Jiaxing black pig pork traits is explored from the molecular level, in order to provide theoretical basis corresponding to improvement ofthe meat quality of local pigs, protection of germplasm resources of Jiaxing black pigs, and development and utilization, and provide great significance for improving fertility of low-yield varietiesand improving meat quality.

Description

(1) Technical field [0001] The invention relates to a characteristic sequence of the TFB2M gene of Jiaxing black pig and specific amplification primers, and a detection kit for quality-related traits of Jiaxing black pork including the characteristic sequence of the TFB2M gene and the specific amplification primer. (2) Background technology [0002] Jiaxing black pig is an excellent local breed in my country. After a long period of domestication and long-term selection by farmers in the production area, it has formed excellent characteristics of high fecundity, good meat quality, strong stress resistance, and good hybrid combination ability, especially fecundity. The high characteristic has attracted the attention of the world, and it is a precious pig breed resource in our country. [0003] However, due to the slow growth rate, low slaughter rate, low slaughter rate, low lean meat rate, and low feed conversion rate of local pig breeds in my country, they cannot adapt to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/11C12Q1/6888
CPCC12N9/1007C12Q1/6888C12Q2600/124C12Q2600/158
Inventor 张金枝宋倩倩吴芬许明曙高海霞韩祝君
Owner ZHEJIANG UNIV
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