Jiaxing black pig TFB2M gene characteristic sequence, primer and detection kit
A technology for detecting kits and genetic characteristics, which is applied in genetic engineering, plant genetic improvement, microbial measurement/inspection, etc., and can solve problems such as low feed conversion rate, low lean meat rate, and shrinking population size
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Embodiment 1
[0033] 1.1 Materials and methods
[0034] 1.1.1 Experimental materials
[0035] 1.1.1.1 Experimental animals
[0036] A healthy Jiaxing black pig of about 72kg was selected, provided by Zhejiang Qinglian Food Co., Ltd. After slaughtering, the livers were taken out, placed in cryopreservation tubes and put into liquid nitrogen tanks for quick freezing. After returning to the laboratory, they were taken out of the liquid nitrogen tanks and stored in a -80°C refrigerator for later use as test samples.
[0037] 1.1.1.2 Main instruments and reagents
[0038] 1.1.1.2.1 Main instruments
[0039]
[0040] 1.1.1.2.2 Main reagents
[0041] Trizol A + Reagents, plasmid small extraction kit (No.DP103), DH5α competent cells (No.CB101), DL2000 Marker DL1000 Marker were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; Prime Script RTreagent Kit with gDNA Eraser (No.DRR037A ), EX Taq (No.DR001A), and Gel Extraction Kit (gel recovery kit) were purchased from Treasure...
Embodiment 2
[0150] Example 2: Analysis of differential expression of TFB2M gene in different tissues of Jiaxing black pig
[0151] 2.1 Materials and methods
[0152] 2.1.1 Sample material
[0153] 2.1.1.1 Test sample
[0154]Choose 10 Jiaxing black pigs that are healthy and reach the market slaughter weight, 5 Berkshire x Jiaxing black pigs, 5 Landrace x Jiaxing black pigs, 5 Du x Bajia pigs, 5 Du x Changjia pigs, and 5 Berkshire pigs. The scissors and tweezers used for tissue collection were sterilized at high temperature before being used for later use. After slaughtering, 8 tissues including heart, liver, spleen, lung, sebum, abdominal fat, stomach, and leg muscles were collected quickly, put them into cryopreservation tubes, quickly put them into a liquid nitrogen tank, and bring them back to the laboratory. Store in -80°C refrigerator for later use.
[0155] 2.1.1.2 Test equipment
[0156] Scissors, tweezers, freezing tubes, liquid nitrogen, freezing boxes, real-time fluorescent...
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