Method and application of bovine serpina3 gene genetic marker-assisted detection of bovine growth and carcass traits

A molecular marker-assisted, gene technology, applied in the field of molecular biology technology and cattle breeding, can solve the problems that have not yet been reported on SERPINA3 gene research.

Active Publication Date: 2022-01-18
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] So far, there is no research report on the association of SERPINA3 gene with growth and carcass traits in Chinese local cattle breeds

Method used

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  • Method and application of bovine serpina3 gene genetic marker-assisted detection of bovine growth and carcass traits
  • Method and application of bovine serpina3 gene genetic marker-assisted detection of bovine growth and carcass traits
  • Method and application of bovine serpina3 gene genetic marker-assisted detection of bovine growth and carcass traits

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[0022] The present invention will be described in detail below in conjunction with the drawings and embodiments, and the embodiments are to explain the present invention rather than limit it.

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Abstract

The invention discloses a method for assisted detection of bovine growth and carcass traits by genetic markers of bovine SERPINA3 gene and its application. Using the whole genome DNA of yellow bovine to be tested as a template, PCR amplifies a partial fragment of the bovine SERPINA3 gene, and performs gene expression by PCR-RFLP technology. Genetic variation detection, the detection method of the present invention is simple, fast, low cost, accurate and reliable detection results, suitable for large-scale genotyping of cattle SERPINA3 gene genetic variation sites, and assisting molecular markers of cattle growth and carcass traits Selection provides new genetic markers, which can speed up the establishment of cattle populations with excellent genetic resources.

Description

technical field [0001] The invention relates to the fields of molecular biology technology and cattle breeding, and relates to the detection of gene mutation sites, in particular to a method and a kit for assisting detection of bovine growth and carcass traits by SNP markers of bovine SERPINA3 gene. Background technique [0002] my country's local yellow cattle breeds are rich in resources, but compared with foreign high-quality cattle breeds, there are problems such as small body size, underdeveloped rear drive, slow growth rate, low slaughter rate and net meat rate. does not have an advantage. Therefore, it is imperative to breed high-quality local cattle breeds in our country, and it is of great significance to promote the development of animal husbandry to breed high-quality local cattle breeds with fast growth rate and high net meat rate. [0003] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a site polymorphism caused by the substitution of a ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 贺花胡志刚刘小林
Owner NORTHWEST A & F UNIV
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