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A Strain of Avian Encephalomyelitis Virus and Its Application

A technology of avian encephalomyelitis, virus, applied in the field of animal virology

Active Publication Date: 2022-05-10
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific treatment for the disease, and it can only be prevented and controlled by live vaccine and inactivated vaccine immunization.

Method used

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  • A Strain of Avian Encephalomyelitis Virus and Its Application
  • A Strain of Avian Encephalomyelitis Virus and Its Application
  • A Strain of Avian Encephalomyelitis Virus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A strain of avian encephalomyelitis virus, the isolation and acquisition process of the virus is as follows:

[0025] In December 2016, in a layer chicken farm of a local breed in Jinan City, a suspected AE infection occurred in the chicken farm. The cloacal cotton swabs of the layer chickens were taken aseptically treated and inoculated with the yolk sac of 6-day-old SPF chicken embryos, and the incubation continued for 12 days. The chickens were observed Embryopathies. The first-generation chicken embryos had no lesions, and the chicken embryo brains were aseptically harvested. After repeated freezing and thawing for 3 times, they were passed on SPF chicken embryos. After 12 days of inoculation, the SPF chicken embryos showed weakened activity, dysplasia, short stature, muscle atrophy, toe deformation and The characteristic lesions of AE such as betel nut liver were preliminarily identified as avian encephalomyelitis virus by animal regression test, and named it AEV J...

Embodiment 2

[0027] Relevant detection and analysis of embodiment 2 virus

[0028] 1. For the detection of virus content, use sterilized normal saline to dilute the virus species 10 times, and take 10 -5 、10 -6 、10 -7 、10 -8 4 dilutions, yolk sac route to inoculate 5-6 day-old SPF chicken embryos, inoculate 0.2ml per embryo. After inoculation, continue to incubate at 37°C, illuminate the eggs every 8 hours, take out the dead embryos in time, and discard the dead chicken embryos within 72 hours, depending on whether the dead chicken embryos after 72 hours or have the following symptoms: chicken embryo development retardation, short stature , brain edema, progressive muscle atrophy, toe deformation, and betel nut liver are AE lesions. The observation results of chicken embryo death and lesions are shown in Table 1. According to the number of chicken embryo lesions, EID is calculated according to the Reed-Muench method 50 , virus content≧10 per 0.2ml 6.75 EID 50 .

[0029] Table 1 EI...

Embodiment 3

[0049] The preparation of embodiment 3 inactivated vaccines

[0050] Use the E3 generation of AEV JN as the seed virus, dilute it with sterilized normal saline or PBS at 1:100, inoculate the yolk sac with 6-day-old SPF chicken embryos, hatch to 16-day-old, place at 2-4°C overnight, and take the ones with obvious lesions Chicken embryos, take the homogenate of the embryo body (discard the eyes and limb bones), make a suspension with PBS or the same batch of allantoic fluid at a ratio of 1:3, freeze and thaw 3-5 times, or use ultrasonic treatment, and place in sterilized In a sterile container, filter it with sterilized four-layer gauze into a sterile container, add 10% formalin to make the final concentration of formaldehyde 0.2%, and inactivate it in a constant temperature shaking incubator at 37°C for 24 hours. After the inactivation test is qualified, it is emulsified according to the ratio of oil to water 2:1 to prepare an oil emulsion inactivated vaccine;

[0051] The vac...

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Abstract

The present invention relates to the field of animal virology, and provides a strain of avian encephalomyelitis virus and its application, specifically provides a strain of avian encephalomyelitis virus, named AEV JN, and its preservation number is: CGMCC No.16385, It also provides the isolation and identification process of the virus strain. Through the genetic evolution analysis of the VP1 gene, it is confirmed that it is in the same branch as the internationally recognized AEV virulent strain VR strain, and has a close relationship. It fills in the relevant gaps in my country. As a vaccine strain, it can well complement the existing 1143 strains of live vaccine commonly used in my country, and provide better protection for my country's poultry breeding industry.

Description

technical field [0001] The invention relates to the field of animal virology, and provides an avian encephalomyelitis virus strain and application thereof. Background technique [0002] Avian encephalomyelitis (AE), also known as epidemic tremor, is a viral infectious disease characterized by ataxia and head and neck tremor caused by avian encephalomyelitis virus (AEV) infecting poultry. Birds of various ages (chicken, quail and turkey, etc.) can be infected through the digestive tract, but the incidence of infection is different. Young chickens infected with the virus can cause paralysis, head and neck tremors and even ataxia, and adult chickens often have subclinical infection or lead to a transient decline in egg production (5%-10%), and the incidence of the disease is generally 40-40%. 60%, with a mortality rate of 25-50%. Since the disease was first discovered in the United States in 1932, it has spread to many countries and regions around the world, causing huge econ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/125A61P31/14C12R1/93
CPCA61P31/14C12N7/00A61K2039/5252A61K2039/552C12N2770/32334C12N2770/32321
Inventor 徐怀英秦卓明高月花王友令袁小远张玉霞杨金兴
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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