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Animal composite immune globulin production method

An immunoglobulin and a production method technology are applied in the production field of compound immunoglobulin for animals, can solve the problems of single immunoglobulin, low product purity, unsatisfactory immune effect, etc., achieve good immune effect and avoid difficult absorption Effect

Active Publication Date: 2006-04-26
DALIAN SANYI ANIMAL MEDICINE CO LTD +2
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0011] The present invention aims to solve the technical problems of single immunoglobulin, unsatisfactory immune effect and low product purity in the prior art, and provides a production of compound immunoglobulin for animals with three kinds of immunoglobulins and high purity. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0056] a. Preparation of immune serum

[0057] (1). Select susceptible homologous animals and immunize with a certain amount of immunogen. The number of immunizations is determined according to different animals and different immunogens;

[0058] (2). Separation of immune serum: place the container containing blood at room temperature for about 1 hour to allow it to fully coagulate, and then place it at 4°C overnight;

[0059] (3). Aspirate the clear serum and place it in the bottle; if the serum contains blood cells or blood clots, centrifuge at 3000g for 20 minutes to collect the upper clear serum;

[0060] (4). Divide the immune serum into appropriate containers and store at -20°C for later use.

[0061] b. Separation and extraction of immunoglobulin IgG, IgA and IgM with immune serum

[0062] (1). Mix the serum with 3 times the volume of water, adjust the pH value to 7.7, put it in a low-temperature water tank (containing 20% ​​ethanol aqueous solution) or an ice-water b...

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Abstract

The production process of composite immune globulin for animal includes the following steps: preparing immune blood serum and packing immune blood serum at -20 deg.c inside storing container; separating immune blood serum to extract immune globulin IgG, IgA and IgM; purifying immune globulin IgG, IgA and IgM; mixing immune globulin IgG, IgA and IgM in the ratio of 8-10 to 1 to 1-2; and nanofiltering. The composite immune globulin thus produced includes three kinds of immune globulin, IgG, IgA and IgM, and has three kinds of immune functions, high immune effect of preventing animal viral diseases and easy absorption.

Description

Technical field: [0001] The invention relates to a production method of animal immunoglobulin, especially a production method of compound immunoglobulin for animal. Background technique: [0002] Animal viral diseases have always been a major problem in the breeding industry, and the annual losses caused by animal viral diseases are shocking. At present, in addition to antiviral drugs, veterinary medicine mostly uses virus-specific hyperimmune serum to treat animal viral diseases. At present, the production method of virus-specific hyperimmune serum is carried out according to the following steps: [0003] 1. Measure a certain amount of animal serum, slowly add an equal volume of 80% supersaturated ammonium sulfate under slow stirring, add the second drop after each drop, stir evenly and put it at 4°C Set aside for 1-2 hours, take it out and centrifuge at 3000-5000 rpm for 15-20 minutes, discard the supernatant, and dissolve the precipitate with normal saline of the same v...

Claims

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Application Information

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IPC IPC(8): C07K16/06A61K39/395A61P31/12
Inventor 江国托
Owner DALIAN SANYI ANIMAL MEDICINE CO LTD
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