Method for auxiliary detection of growth and carcass traits of cattle by heritable markers of cattle SERPINA3 gene and application thereof

A gene and genotype technology, applied in the field of cattle SERPINA3 gene SNP marker-assisted detection of cattle growth and carcass traits, can solve the problems that have not yet been reported on SERPINA3 gene research

Active Publication Date: 2019-02-01
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] So far, there is no research report on the association of SE...

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  • Method for auxiliary detection of growth and carcass traits of cattle by heritable markers of cattle SERPINA3 gene and application thereof
  • Method for auxiliary detection of growth and carcass traits of cattle by heritable markers of cattle SERPINA3 gene and application thereof
  • Method for auxiliary detection of growth and carcass traits of cattle by heritable markers of cattle SERPINA3 gene and application thereof

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[0022] The present invention will be described in detail below in conjunction with the drawings and embodiments, and the embodiments are to explain the present invention rather than limit it.

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Abstract

The invention discloses a method for auxiliary detection of growth and carcass traits of cattle by heritable markers of a cattle SERPINA3 gene and an application thereof. A whole genome DNA of the cattle to be tested is used as a template, a part of fragments of the cattle SERPINA3 gene are amplified by PCR, and genetic heritable variation detection is performed by a PCR-RFLP technology. The detection method is simple and fast, low in cost, and accurate and reliable in detection results, is suitable for large-scale genotyping of genetic heritable mutation loci of the cattle SERPINA3 gene, provides new heritable markers for molecular marker assistant selection of the growth and carcass traits of the cattle, thereby speeding up the establishment of cattle populations with excellent heritableresources.

Description

technical field [0001] The invention relates to the fields of molecular biology technology and cattle breeding, and relates to the detection of gene mutation sites, in particular to a method and a kit for assisting detection of bovine growth and carcass traits by SNP markers of bovine SERPINA3 gene. Background technique [0002] my country's local yellow cattle breeds are rich in resources, but compared with foreign high-quality cattle breeds, there are problems such as small body size, underdeveloped rear drive, slow growth rate, low slaughter rate and net meat rate. does not have an advantage. Therefore, it is imperative to breed high-quality local cattle breeds in our country, and it is of great significance to promote the development of animal husbandry to breed high-quality local cattle breeds with fast growth rate and high net meat rate. [0003] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a site polymorphism caused by the substitution of a ...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 贺花胡志刚刘小林
Owner NORTHWEST A & F UNIV
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