Lung adenocarcinoma diagnostic composition and application thereof
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A composition and technology of lung adenocarcinoma, applied in the field of disease diagnosis, can solve the problem that the diagnosis of lung cancer cannot meet the clinical needs
Inactive Publication Date: 2019-11-12
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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At present, there are tumor markers (tumor marker, TM) widely used clinically, but the diagnosis of lung cancer using only one marker cannot meet the clinical needs [Linjie L, Jinlong T, Lijun Z, et al. The Combination of the Tumor Markers Suggests the Histological Diagnosis of Lung Cancer[J].BioMed Research International,2017,2017:1-9]
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Embodiment 1
[0050] Example 1 Detection of differentially expressed genes in lung adenocarcinoma tissue
[0051] 1. Collection of samples
[0052] 59 lung adenocarcinoma tissues and 59 adjacent tissues were collected, and all samples were placed in cryopreservation tubes with RNA protection solution immediately after isolation, and stored in a -80°C refrigerator within 30 minutes. The entire operation and storage The process follows the enzyme-free principle.
[0053] 2. Preparation of RNA samples
[0054] Total RNA was extracted from lung adenocarcinoma tissues and paracancerous tissues by TRIzol RNA extraction method.
[0055] (1) Pre-cool the mortar with liquid nitrogen, put the tissue sample into the mortar with liquid nitrogen, and fully grind the tissue sample into powder under liquid nitrogen;
[0056] (2) Transfer the sample powder into a 2.0ml EP tube filled with TRIzol lysate, (50mg of tissue sample can be added to each ml of lysate), shake vigorously, mix well, and lay flat a...
Embodiment 2
[0095] Example 2 Candidate gene ROC curve analysis
[0096] 1. ROC curve analysis
[0097] Use the pROC package of R language to analyze the receiver operating characteristics of PLPP4, HHIPL2, B3GNT6, INHA, ECEL1 or ALAS2, calculate the binomial exact confidence space, draw the ROC curve, and calculate the area under the curve.
[0098] 2. Results
[0099] As shown in Table 1, compared with the AUC values of PLPP4, HHIPL2, B3GNT6, INHA or ECEL1 single genes, the AUC values of any combination among PLPP4, HHIPL2, B3GNT6, INHA or ECEL1 genes were increased, suggesting that the above four At least two of the genes can be used in combination for the diagnosis of lung adenocarcinoma.
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Abstract
The present invention discloses a lung adenocarcinoma diagnostic composition and an application thereof. The composition comprises an agent for detecting at least two genes of PLPP4, HHIPL2, B3GNT6, INHA or ECEL1. The present invention discloses the application of the lung adenocarcinoma diagnostic composition in preparing a lung adenocarcinoma diagnostic product or kit. Experiments prove that expression levels of the PLPP4, HHIPL2, B3GNT6, INHA or ECEL1 in lung adenocarcinoma tissues are significantly higher than those in para-carcinoma tissues, at the same time, an AUC value of a combined application of the PLPP4, HHIPL2, B3GNT6, INHA or ECEL1 is higher, accuracy is relatively high, and the lung adenocarcinoma diagnostic composition provides an effective diagnostic indicator for clinicaldiagnosis of lung adenocarcinoma.
Description
technical field [0001] The invention belongs to the field of disease diagnosis and relates to a lung adenocarcinoma diagnostic composition and its application, in particular to the application of PLPP4, HHIPL2, B3GNT6, INHA or ECEL1 in combined diagnosis of lung adenocarcinoma. Background technique [0002] Currently, the incidence of lung adenocarcinoma is increasing year by year [Shen J, Wang B, Zhang T, Zhu N, Wang Z, Jin J, He Y and Hu M: Suppression of non-small cell lung cancer growth and metastasis by a novel small molecular activator of RECK. Cell Physiol Biochem 45:1807-1817, 2018]. The diagnosis of lung adenocarcinoma mainly relies on symptoms, signs, laboratory tests, imaging tests, and pathological biopsy, including bronchoscopy, needle aspiration cytology, and other biopsies. [0003] At the same time, the diagnosis of lung adenocarcinoma needs to combine the patient's medical history, laboratory and imaging examinations, histopathological biopsy and immunohist...
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