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KASP marker relevant with wheat head length under low-salt condition and application of KASP marker

A wheat and marker technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of high cost of high-density SNP chip technology

Active Publication Date: 2019-12-03
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the SNP marker linked to the target trait is obtained through linkage analysis or association analysis, and other materials contain the excellent locus, the cost of simplified genome sequencing or high-density SNP chip technology is obviously too high, and it is unnecessary

Method used

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  • KASP marker relevant with wheat head length under low-salt condition and application of KASP marker
  • KASP marker relevant with wheat head length under low-salt condition and application of KASP marker
  • KASP marker relevant with wheat head length under low-salt condition and application of KASP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 The discovery of wheat ear length-related SNP markers under low-salt conditions and the development of KASP markers and the establishment of detection methods

[0054] 1. Phenotypic identification of wheat ear length under low-salt conditions

[0055] 191 varieties (lines) selected from the collected wheat germplasm resources are used as research materials. The geographical sources include 14 provinces and autonomous regions, including 17 in Beijing, 40 in Hebei, 30 in Henan, 34 in Shandong, and 8 in Shanxi. , 39 in Shaanxi, 6 in Qinghai, 3 in Gansu, 1 in Jilin, 1 in Inner Mongolia, 1 in Tibet, 1 in Tianjin, 1 in Anhui, 3 in Sichuan, in addition to 5 imported species and 1 unknown Source wheat germplasm.

[0056] During the three years of 2014-2015, 2015-2016 and 2016-2017, the ear length of 191 wheat varieties (lines) were tested at the Nanpi Ecological Agriculture Experimental Station of the Chinese Academy of Sciences (116°40′E, 38°00′N, 11m above sea leve...

Embodiment 2

[0112] Example 2 Verification of SNP marker AX-111449179 in double haploid population

[0113] In 2017-2018, under low-salt conditions, the ear length traits of 149 lines and their two parents of the DH population (Hanxuan 10 × Lumai 14) were identified. The experiment was carried out at the Nanpi Ecological Agriculture Experimental Station of the Chinese Academy of Sciences (116°40′E, 38°00′N, 11m above sea level), and the salt content of the field soil was 0.18% (m / m). The experiment adopts random block design, and each line is planted in a three-row block with a row length of 2m, a row spacing of 20cm, and a plant spacing of 10cm. Ensure that after emergence, there is no irrigation during the growth period, conventional management in the field, and no serious pests and diseases and lodging during the growth period. Five individual plants with the same growth in the middle row were harvested from each line. Measure the length of the main ear.

[0114] According to the sli...

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Abstract

The invention discloses a KASP marker relevant with wheat head length under low-salt condition and an application of the KASP marker, and provides an application of a substance for detecting polymorphism or genotype of an SNP marker AX-111449179 to identification or auxiliary identification of the wheat head length. The SNP marker AX-111449179 is 22745792rd nucleotide on 7A chromosome on the wheat, namely C or T. The invention develops the SNP marker AX-111449179 associated KASP marker relevant with the wheat head length under low-salt condition, and a molecule auxiliary selection means is provided for establishment of a theoretical basis for breeding of salt-tolerant wheat varieties being high and stable in yield and good in quality.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a KASP marker related to wheat ear length under low-salt conditions and an application thereof. Background technique [0002] Wheat is one of the most important food crops in the world, and about 35% of the world's population uses wheat as a staple food. China is a major wheat producer and consumer, and wheat production plays a vital role in China's food security. The total area of ​​salinized soil in China is 1.487 billion mu (approximately 100 million hectares). How to use the large area of ​​saline-alkali land and other low-yield fields to effectively increase the total wheat yield is a major problem that needs to be solved urgently. Wheat panicle traits have direct or indirect effects on wheat yield. Wheat panicle length is a quantitative trait controlled by multiple genes. Compared with other panicle traits such as spikelet number and panicle density, the heritability of wheat...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 郑琪胡盼罗巧玲杨国堂李滨李宏伟李振声
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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