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A kind of bifunctional graphene quantum dot and its preparation method and application

A dual-function, quantum dot technology, applied in the field of biosensors, can solve the problems that the detection results are easily affected by the detection environment, increase the biotoxicity of carbon dots, and the instability of electrochemical signals, and achieve strong water solubility and good fluorescence quenching efficiency , highly specific effect

Active Publication Date: 2021-03-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] It has been reported in the prior art that sulfur and nitrogen co-doped carbon dots are used as luminous bodies and reaction accelerators to construct an electrochemical luminescence system for detecting the content of atrazine in water, but due to the high sensitivity of the electrochemical sensing system itself, The detection results are easily affected by the detection environment, which makes the electrochemical signal unstable.
There is also an existing technology that uses silver nano-ions to modify carbon dots as a substrate for surface-enhanced Raman resonance. The SERS signal of rhodamine 6G is enhanced through the aggregation of target-induced carbon dots to quantitatively detect atrazine. However, the silver nano-ions The introduction increases the biotoxicity of carbon dots and weakens their biocompatibility, so it is no longer suitable for the determination of atrazine content in organisms
The prior art has also reported the successful quantitative detection of acetamiprid content in real samples by using the inner filter effect between gold nanoparticles and carbon dots, but the detection sensitivity is not enough

Method used

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  • A kind of bifunctional graphene quantum dot and its preparation method and application
  • A kind of bifunctional graphene quantum dot and its preparation method and application
  • A kind of bifunctional graphene quantum dot and its preparation method and application

Examples

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Embodiment 1

[0046] The preparation of embodiment 1DPA-GQD-His

[0047] Take a certain amount of citric acid monohydrate in a beaker, add water to ultrasonically dissolve, then weigh D-penicillamine and Histidine was dissolved in a beaker and sonicated. After the dissolution is complete, quantitatively transfer to a high-pressure reactor, place in a blast drying oven, and react for 1 hour at 200°C. After the reaction, the pH of the obtained solution was adjusted to neutral, and then placed in a 3.5KD dialysis bag for dialysis for 48 hours. After the dialysis, the solution in the bag was taken and freeze-dried to obtain D-penicillamine-histidine bifunctional graphene quantum dots (DAP-GQD-His) solid powder. The D-penicillamine-histidine double graphene quantum dot prepared in this embodiment is characterized, by figure 1 The TEM image shows that the D-penicillamine-histidine bifunctional graphene quantum dot DPA-GQD-His is composed of 2D graphene sheets with a size between 1-6nm and an a...

Embodiment 2

[0051] The preparation of embodiment 2DPA-GQD-His

[0052] When the reaction temperature in Example 1 is 200°C, and the reaction time is 1h, control citric acid: the molar ratio of histidine is 1:1, adjust the molar ratio of citric acid and D-penicillamine, so that D-penicillamine The molar ratios of mycamine to citric acid are respectively 0.1:1, 0.2:1, 0.3:1, 0.4:1, 0.5:1, 0.6:1, 0.7:1, and the rest of the operation steps are consistent with Example 1, and different DPA-GQD-His D-penicillamine-histidine bifunctional graphene quantum dots.

[0053] Measure the fluorescence intensity of the prepared DPA-GQD-His, the results are as follows Image 6 as shown, Image 6 The middle line from bottom to top corresponds to the preparation of D-penicillamine and citric acid with a molar ratio of 0.1:1, 0.2:1, 0.3:1, 0.7:1, 0.4:1, 0.6:1, and 0.5:1, respectively. The resulting fluorescence intensity of DPA-GQD-His. It can be found that with the increase of D-penicillamine incorporati...

Embodiment 3

[0054] The preparation of embodiment 3DPA-GQD-His

[0055] When the reaction temperature in Example 1 is 200°C, and the molar ratio of citric acid, histidine, and D-penicillamine is 1:1:0.5, the time for controlling the hydrothermal reaction is 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, and the rest of the operation steps were the same as in Example 1, and different DPA-GQD-His D-penicillamine-histidine bifunctional graphene quantum dots were prepared.

[0056] Measure the fluorescence intensity of the prepared DPA-GQD-His, the results are as follows Figure 7 as shown, Figure 7 The middle lines from bottom to top correspond to the fluorescence intensities of DPA-GQD-His prepared with reaction times of 6h, 5h, 4h, 3h, 0.5h, 2h, and 1h, respectively. It can be found that the fluorescence intensity of DPA-GQD-His reaches the maximum when the reaction time is 1 h.

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Abstract

The invention discloses bifunctional graphene quantum dots, and a preparation method and an application thereof, and belongs to the technical field of biosensors. The preparation method comprises thesteps: according to the molar ratio of citric acid to histidine to D-penicillamine of 1:0.1:0.01-1:1:1, preparing the dual-functionalized graphene quantum dots DPA-GQD-His by a hydrothermal method. The bifunctional graphene quantum dots provided by the invention can quantitatively detect one or more organophosphorus pesticides with high sensitivity and high selectivity, and the preparation methodis simple and easy to popularize.

Description

technical field [0001] The invention relates to a bifunctional graphene quantum dot and its preparation method and application, belonging to the technical field of biosensors. Background technique [0002] Organophosphorus pesticides are typical neurotoxins, and those skilled in the art generally believe that the toxic mechanism of organophosphorus pesticides is: the combination of organophosphorus pesticides and acetylcholine (ACh) in cholinergic synapses, thereby inhibiting acetylcholinesterase (AChE ) activity, making it unable to hydrolyze ACh, resulting in a large accumulation of ACh in the synaptic cleft, resulting in toxic effects, which is also the main insecticidal mechanism of organophosphorus pesticides. Common exposure routes for acute poisoning of organophosphorus pesticides include skin contact, oral administration, and inhalation. The incubation period ranges from 30 minutes to 2 hours. Common clinical symptoms include dizziness, nausea, vomiting, salivation, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/184G01N21/64B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C01B2204/04C01B2204/32C01B32/184G01N21/643G01N2021/6432
Inventor 李在均李娜娜李瑞怡
Owner JIANGNAN UNIV
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