New drug for preventing and treating mythimna separata and use method thereof
A new drug and armyworm technology, applied in the fields of botanical equipment and methods, application, pesticides, etc., can solve the problem of lack of new pesticides and active molecules for the prevention and control of armyworms, etc., and achieve the effect of good insecticidal activity
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[0022] Embodiment: A kind of new medicine of preventing and treating armyworm and its application method
[0023] 1.1 Armyworms tested
[0024] Armyworm 2nd instar larvae.
[0025] 1.2 Cyclosporin A concentration
[0026] Cyclosporin A doses: 50 μM, 100 μM and 150 μM.
[0027] 1.3 Bioassay methods
[0028] In this paper, medicament mixed artificial feed (yeast powder 34 g, wheat germ powder 40 g, corn leaf powder 100 g, mix well; 2, sorbic acid 2 g, methylparaben 4 g, 300 mL distilled water boiled and mixed well Finally, add the mixture to ① and mix well; 3. 600mL distilled water, 18 g agar powder, boil and mix well, then add to ②, stir evenly; 4. When the temperature drops to 40 ℃, add VC 2.5 g, linoleic acid (Soybean oil) 5-6 drops, mix well; store at 5, 4 ℃.) method for bioassay. That is, the armyworm artificial diet was prepared first, and before the feed was cooled and solidified, different doses of cyclosporine A were mixed into the armyworm artificial diet, and the...
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