A new drug for preventing and treating armyworm and its application method
A technology for the prevention and control of armyworms and pesticides, which is applied in the field of toxicological activity of pest control, can solve the problems of lack of new pesticides and active molecules for the control of armyworms, and achieve good insecticidal effects
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[0022] Embodiment: A kind of new medicine of preventing and treating armyworm and its application method
[0023] 1.1 Armyworms tested
[0024] Armyworm 2nd instar larvae.
[0025] 1.2 Cyclosporin A concentration
[0026] Cyclosporin A doses: 50 μM, 100 μM and 150 μM.
[0027] 1.3 Bioassay methods
[0028] In this paper, medicament mixed artificial feed (yeast powder 34 g, wheat germ powder 40 g, corn leaf powder 100 g, mix well; 2, sorbic acid 2 g, methylparaben 4 g, 300 mL distilled water boiled and mixed well Finally, add the mixture to ① and mix well; 3. 600 mL distilled water, 18 g agar powder, boil and mix well, add to ②, and stir evenly; 4. When the temperature drops to 40 ℃, add VC2.5 g, sub 5-6 drops of oleic acid (soybean oil), mix well; store at 5, 4°C.) for bioassay. That is, the armyworm artificial diet was prepared first, and before the feed was cooled and solidified, different doses of cyclosporine A were mixed into the armyworm artificial diet, and then th...
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