Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of composition of donkey-hide gelatin and novel donkey-hide gelatin to preparation of drug for preventing or treating diseases related to low leucocyte amount

A white blood cell and composition technology, which is applied in the direction of extracellular fluid diseases, blood diseases, drug combinations, etc., can solve the problems of drug application that does not disclose the combination of donkey-hide gelatin and new donkey-hide gelatin to reduce related diseases, and the use of new donkey-hide gelatin has limited effects

Pending Publication Date: 2020-01-17
山东福牌阿胶股份有限公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual use, it will be found that the use of donkey-hide gelatin and new donkey-hide gelatin has limited effects, and the prior art does not disclose the application of donkey-hide gelatin and new donkey-hide gelatin compositions in the preparation of drugs for the prevention or treatment of diseases related to leukopenia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of composition of donkey-hide gelatin and novel donkey-hide gelatin to preparation of drug for preventing or treating diseases related to low leucocyte amount
  • Application of composition of donkey-hide gelatin and novel donkey-hide gelatin to preparation of drug for preventing or treating diseases related to low leucocyte amount
  • Application of composition of donkey-hide gelatin and novel donkey-hide gelatin to preparation of drug for preventing or treating diseases related to low leucocyte amount

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Tolerance test of donkey-hide gelatin and new donkey-hide gelatin composition:

[0021] The experimental method is as follows: the composition of donkey-hide gelatin and new donkey-hide gelatin is first ground into powder, and then dissolved in sterile water to prepare different concentrations of experimental groups. When the zebrafish fertilized eggs developed to 24hpf, select the normally developed zebrafish fertilized eggs, move them into a 6-well plate, add 25 fertilized eggs to each well, and set the concentration (μg / ml) of 8 groups of experimental groups: 6.25, 12.5, 25, 50, 100, 200, 400, 800 and the blank control group (embryo culture water), two replicate wells were set up for each concentration group, and then incubated in a light incubator at a constant temperature (28°C) for 3 days of continuous administration. The medium was changed every 24 hours, and the survival rate and hatching rate at each time point were recorded.

[0022] The specifi...

Embodiment 2

[0027] Example 2: In this example, experiments were conducted to verify the reversal effect of the combination of donkey-hide gelatin and new donkey-hide gelatin on leukopenia in zebrafish induced by chloramphenicol.

[0028] Experimental methods include:

[0029] S1: Transgenic zebrafish embryos with green fluorescent immune cells at 2dpf were selected for experimentation.

[0030] S2: Drug application: the hatched transgenic zebrafish embryos were placed in chloramphenicol and different concentrations of donkey-hide gelatin and new donkey-hide gelatin composition solutions for 24 hours. There were 12 larvae in each experiment, and 3 parallel wells were set up in each group.

[0031] S3: Observation: After 24 hours of treatment with the donkey-hide gelatin and new donkey-hide gelatin composition, observe the number of green fluorescent cells in the zebrafish larvae under a fluorescent microscope, take pictures, and perform statistical analysis.

[0032] The specific impleme...

Embodiment 3

[0039] Example 3: In this example, a verification experiment was conducted on the recovery effect of the combination of Ejiao and new Ejiao on the reduction of white blood cells in zebrafish caused by vinorelbine.

[0040] Experimental methods include:

[0041] S1: Transgenic zebrafish embryos with green fluorescent platelets at 2dpf were selected for the experiment.

[0042] S2: Drug application: the hatched transgenic zebrafish embryos were placed in vinorelbine and different concentrations of donkey-hide gelatin and new donkey-hide gelatin composition solutions for 24 hours. In each group of experiments, 12 larvae were tested, and 3 parallel wells were set up in each group.

[0043] S3: Observation: After 24 hours of treatment with the donkey-hide gelatin and new donkey-hide gelatin composition, observe the number of green fluorescent cells in the zebrafish larvae under a fluorescent microscope, take pictures, and make statistics.

[0044] The specific implementation condit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a composition of donkey-hide gelatin and novel donkey-hide gelatin to the preparation of a drug for preventing or treating diseases related to low leucocyte amount. A large number of experiments find that the composition of the donkey-hide gelatin and the novel donkey-hide gelatin has a reversing effect on chloramphenicol or vinorelbine-induced low leucocyteamount of zebrafish, and the effect of reversing the low leucocyte amount by the composition of the donkey-hide gelatin and the novel donkey-hide gelatin at a certain ratio and action concentration isfar greater than the effect of using only donkey-hide gelatin or only applying the novel donkey-hide gelatin. The experiment probes that the composition of the donkey-hide gelatin and the novel donkey-hide gelatin can be used for preparation of the drug for preventing or treating the diseases related to the low leucocyte amount, particularly for preparation of a drug for clinically related diseases caused by low leucocyte amount in a body, including leukopenia caused by radiotherapy and chemotherapy.

Description

technical field [0001] The present application relates to a new application of a donkey-hide gelatin composition, in particular to an application of a donkey-hide gelatin and a new donkey-hide gelatin composition in the preparation of medicines for preventing or treating diseases related to leukopenia. Background technique [0002] Low white blood cells in cancer patients are mostly caused by anti-tumor treatment. Patients receiving chemotherapy often have leukopenia, which is because the chemotherapy of chemotherapy drugs acts on different links of leukocyte production and apoptosis, resulting in leukopenia. In patients receiving radiation therapy, ionizing radiation affects all bone marrow components, so leukopenia is also common. However, after receiving multiple radiotherapy and chemotherapy, the bone marrow reserve function is poor, and it is more prone to leukopenia, or even severe leukopenia. The risk of wound bleeding increases when the white blood cells are lower ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/36A61P7/00
CPCA61K35/36A61P7/00A61K2300/00
Inventor 杨铧杨福安武勇李笃军
Owner 山东福牌阿胶股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com