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Application of colla corii asini and new colla corii asini composition to preparation of medicines for preventing or treating diseases relevant to blood platelet reduction

A technology for platelets and compositions, which is applied to the application field of Ejiao and Xin Ejiao composition in the preparation of medicines for preventing or treating diseases related to thrombocytopenia, and can solve the application of Ejiao and the new Ejiao composition and the use effect of the new Ejiao that are not disclosed. limited issues

Pending Publication Date: 2020-01-24
山东福牌阿胶股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual use, it will be found that the use of donkey-hide gelatin and new donkey-hide gelatin has limited effects, and the prior art does not disclose the application of donkey-hide gelatin and new donkey-hide gelatin compositions in the preparation of drugs for the prevention or treatment of diseases related to thrombocytopenia

Method used

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  • Application of colla corii asini and new colla corii asini composition to preparation of medicines for preventing or treating diseases relevant to blood platelet reduction
  • Application of colla corii asini and new colla corii asini composition to preparation of medicines for preventing or treating diseases relevant to blood platelet reduction
  • Application of colla corii asini and new colla corii asini composition to preparation of medicines for preventing or treating diseases relevant to blood platelet reduction

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: Tolerance test of donkey-hide gelatin and new donkey-hide gelatin composition

[0022] The experimental method in the present embodiment comprises: when zebrafish fertilized egg develops to 24hpf, select the zebrafish fertilized egg of normal development, move into 6 well plates, 25 per hole, set several experimental groups and control groups (embryo culture water ), two replicate wells were set up for each experimental group, and then incubated in a light incubator at a constant temperature (28°C), administered continuously for 3 days, and the medium was changed every 24 hours, and the survival rate and hatching rate at each time point were recorded. The specific implementation conditions and characterization results are shown in Table 1 below.

[0023] Concrete implementation conditions and experimental results in each embodiment of table 1

[0024]

[0025] From the experimental data in Table 1 above, it can be seen that when zebrafish juveniles wer...

Embodiment 2

[0027] Example 2: In this example, the reversal effect of the composition of donkey-hide gelatin and new donkey-hide gelatin on chloramphenicol-induced zebrafish thrombocytopenia is explored. The specific implementation conditions and characterization results are as follows in Table 2:

[0028] Experimental methods include:

[0029] (1) Transgenic zebrafish embryos with green fluorescent platelets at 2dpf were selected for the experiment.

[0030] (2) Drug application: the hatched transgenic zebrafish embryos were treated with chloramphenicol (150 μg / ml) and different concentrations of donkey-hide gelatin and new donkey-hide gelatin compositions for 24 hours. Each experimental group included 12 larvae, and three parallel wells were set up for each group.

[0031] (3) Observation: After 24 hours of treatment with the donkey-hide gelatin and new donkey-hide gelatin compositions, observe the number of green fluorescent cells in the juvenile zebrafish under a fluorescent microsco...

Embodiment 3

[0038] Example 3: The recovery effect of donkey-hide gelatin and new donkey-hide gelatin composition on zebrafish thrombocytopenia caused by vinorelbine. The specific implementation conditions and characterization results are as follows in Table 3:

[0039] Experimental methods include:

[0040] (1) Transgenic zebrafish embryos with green fluorescent platelets at 2dpf were selected for the experiment.

[0041](2) Drug application: the hatched transgenic zebrafish embryos were placed in vinorelbine (100 μg / ml) and different concentrations of donkey-hide gelatin and new donkey-hide gelatin composition solutions for 24 hours. In fact, 12 larvae were included in each group of experiments, and 3 parallel wells were set up in each group.

[0042] (3) Observation: After 24 hours of treatment with the donkey-hide gelatin and new donkey-hide gelatin compositions, observe the number of green fluorescent cells in the juvenile zebrafish under a fluorescent microscope, take pictures, and ...

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Abstract

The invention discloses an application of colla corii asini and a new colla corii asini composition to preparation of medicines for preventing or treating diseases relevant to blood platelet reduction. 2dpf (day post fertilization) blood platelet transgenic zebra fish are used as an experimental animal model, the influence of the colla corii asini and the new colla corii asini composition on bloodplatelets is researched. Under the experimental condition, the colla corii asini and the new colla corii asini composition have reversing effects on blood platelet reduction of zebra fish, induced bychloramphenicol and vinorelbine, and the quantity of the blood platelets can be increased. The experimental result indicates that the colla corii asini and the new colla corii asini composition can be used for preparing medicines for clinically preventing or treating the diseases relevant to the blood platelet reduction, and can be particularly used for preparing the medicines clinically used forrelevant diseases caused by reduction of in vivo blood platelets including low blood platelet diseases caused by chemoradiotherapy.

Description

technical field [0001] The present application relates to a new application of a donkey-hide gelatin composition, in particular to an application of a donkey-hide gelatin and a new donkey-hide gelatin composition in the preparation of a drug for preventing or treating diseases related to thrombocytopenia. Background technique [0002] Low platelets in tumor patients are mostly caused by anti-tumor treatment. Patients receiving chemotherapy often have thrombocytopenia, which is because chemotherapy drugs act on different links of platelet production and apoptosis, resulting in thrombocytopenia. Thrombocytopenia is also common in patients receiving radiation therapy, in which ionizing radiation affects all bone marrow components. After receiving multiple radiotherapy and chemotherapy, the bone marrow reserve function is poor, and thrombocytopenia is more likely to occur, and even severe thrombocytopenia. The risk of wound bleeding increases when the platelet count is lower t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/36A61P7/04
CPCA61K35/36A61P7/04A61K2300/00
Inventor 杨铧杨福安武勇王璐
Owner 山东福牌阿胶股份有限公司
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