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Acacia melanoxylon difficult-to-root clone tissue-cultured seedling domestication process

A technology of tissue culture seedlings and clones, which is applied in the field of domestication technology of tissue culture seedlings of the hard-to-root clones of Acacia blackwood, to achieve the effects of saving running water and ventilation, efficient cultivation, and being beneficial to water retention and rooting

Inactive Publication Date: 2020-01-31
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, in order to solve the problem of transplanting and surviving the hard-to-root clone tissue culture seedlings of Acacia blackwood, the object of the present invention is to provide a kind of domestication process of the hard-to-root clone tissue-culture seedlings of Acacia blackwood. Carry out special research on the domestication of tissue culture seedlings, so that the survival rate and rooting rate can reach 100%, which greatly improves the quality of seedlings, which is of great significance to improving production efficiency, and can also provide reference for transplanting other difficult-to-root tissue culture seedlings

Method used

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  • Acacia melanoxylon difficult-to-root clone tissue-cultured seedling domestication process
  • Acacia melanoxylon difficult-to-root clone tissue-cultured seedling domestication process
  • Acacia melanoxylon difficult-to-root clone tissue-cultured seedling domestication process

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0032] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, after 14 / 18 days of media rooting induction, the rooting rate is only 23.6% ( figure 2 ), the culture medium takes 1 / 2MS as the basic culture medium, adds IBA1.0mg, white sugar 25g, agar 5g, pH value adjustment value 5.5 in every liter of basic culture medium, after the culture medium is subpackaged, it is prepared in advance at 1.1kg / cm 2 Sterilization under high pressure and high temperature;

[0033] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0034] 3) Cultivation in the culture room: place it in the culture room for 15 days after plant...

Embodiment 2

[0040] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0041] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, through 21 days medium rooting induction, the rooting rate is only 36.2% ( Figure 4 ), the culture medium takes 1 / 2MS as the basic culture medium, adds IBA1.5mg, white sugar 30g, agar 6g, pH value adjustment value 6.0 in every liter of basic culture medium, after the culture medium is subpackaged, it is prepared in advance at 1.2kg / cm 2 Sterilization under high pressure and high temperature;

[0042] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0043]3) Cultivation in the culture room: place in the culture room for 20 days after planting, th...

Embodiment 3

[0049] A kind of domestication process of the hard-to-root clone tissue culture seedling of Acacia blackwood:

[0050] 1) Cultivate rooting induced sprouts: select Acacia blackwood clone tissue culture seedlings, through 18 days medium rooting induction, the rooting rate is only 29.3%, the medium is basic medium with 1 / 2MS, in every liter of basic medium Add 1.3mg of IBA, 22g of white sugar, 5.5g of agar, and adjust the pH value to 5.5. 2 Sterilization under high pressure and high temperature;

[0051] 2) Planting sprouts: Take out the sprouts after rooting induction from the culture bottle, rinse them with clean water, and plant them in a sponge-supported seedling tray. When planting, soak the sponge block, fill the bottom layer with 300ml of pure water, and cover it;

[0052] 3) Cultivation in the culture room: place in the culture room for 20 days after planting, the temperature of the culture room is 26°C, and 1700Lx artificial light is added during the day for 11 hours. ...

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Abstract

The invention belongs to the technical field of plant domestication transplanting and particularly relates to an acacia melanoxylon difficult-to-root clone tissue-cultured seedling domestication process. The process includes steps: 1) culturing and rooting induction of bud seedlings, to be specific, selecting acacia melanoxylon difficult-to-root clone tissue-cultured seedlings, and performing rooting induction with a culture medium to obtain rooting induced bud seedlings; 2) bud seedling planting, to be specific, planting the bud seedlings in a sponge supported seedling cultivation tray, soaking sponge in planting, filling the bottom layer with purified water, and covering with a cover; 3) culturing in a culturing room, to be specific, putting the seedling cultivation tray in the culturingroom, opening the cover for ventilation for 10-20min in the morning and evening every day in a medium cultivation stage; 4) culturing in a solar greenhouse, to be specific, transferring the seedlingcultivation tray to a solar greenhouse, keeping the cover of the seedling cultivation tray open all day long, and atomizing once in the morning and evening every day. Therefore, the transplanting survival rate and the rooting rate of the difficult-to-root clone tissue-cultured seedlings can be greatly increased and reach 100%, quality of the seedlings is improved, nursery cultivation time is shortened, nursery management cost is saved, and production efficiency of the acacia melanoxylon tissue-cultured seedlings is improved.

Description

technical field [0001] The invention belongs to the technical field of plant domestication and transplantation, and in particular relates to a domestication process of tissue-cultured seedlings of the hard-to-root clone of Acacia blackwood. Background technique [0002] Acacia melanoxylon is a tree species of Acacia in Mimosaceae, one of the tallest trees in Acacia, native to Australia, Papua New Guinea and Indonesia. Acacia black wood has beautiful wood grain, good material, nitrogen-fixing root nodules, rich litter, and good soil improvement performance. After being introduced to my country in the 1990s, it was successfully planted in Guangdong, Fujian, Guangxi, Hainan and other places. [0003] Over the past century, our unit has overcome the problems of many branches and indistinct trunks through clonal breeding. Acacia blackwood has become a fast-growing, high-yield, precious timber and ecological public welfare tree species in South China. According to incomplete stat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 裘珍飞范春节曾炳山叶森辉冯家勇
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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