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Application of detection reagent with MAFG-AS1 as target site to preparation of drugs for treating bladder cancer

A technology of 1.MAFG-AS1 and 5.MAFG-AS1, applied in the field of biomedicine, can solve the problems of easy metastasis and recurrence of bladder cancer

Active Publication Date: 2020-02-11
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although significant progress has been made in the treatment of bladder cancer in recent years, the problem of easy metastasis and recurrence of bladder cancer still needs to be further solved

Method used

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  • Application of detection reagent with MAFG-AS1 as target site to preparation of drugs for treating bladder cancer
  • Application of detection reagent with MAFG-AS1 as target site to preparation of drugs for treating bladder cancer
  • Application of detection reagent with MAFG-AS1 as target site to preparation of drugs for treating bladder cancer

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Embodiment Construction

[0016] Below in conjunction with accompanying drawing and experimental data, the present invention is further explained and illustrated

[0017] 1. Materials and methods

[0018] Cell culture and transfection, qRT-PCR analysis, Western blot analysis, immunohistochemical assay, MTT assay, immunoprecipitation, immunofluorescence, cell scratch repair experiment, colony formation assay, the above methods are all existing methods, and will not be discussed here. Let me tell you more.

[0019] , patient tissue samples

[0020] In this study, 30 patients with histopathologically diagnosed bladder cancer (BUC) who underwent radical cystectomy (RC) from the Third Xiangya Hospital of Central South University and the Cancer Hospital Affiliated to Xiangya School of Medicine, Central South University from 2016 to 2018 were used. Buried specimens (Table 1). Six radical cystectomy (RC) BUC tissue specimens and corresponding adjacent non-neoplastic tissue specimens were immediately stored ...

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Abstract

The research of the invention shows that lncRNA MAFG-AS1 is significantly overexpressed in primary BUC tissue, and the expression of MAFG-AS1 is up-regulated or down-regulated to enhance or inhibit the proliferation, metastasis and invasion of BUC cells in vitro and in vivo, respectively. Therefore, MAFG-AS1 can be used as a target site in the preparation of drugs for the treatment of bladder cancer. The mechanism study shows that MAFG-AS1 can directly bind to HuR and positively regulate HuR / PTBP1 axis to promote the proliferation, metastasis and invasion of BUC cells. Therefore, MAFG-AS1 canbe used in the preparation of targeted negative regulation reagent of HuR / PTBP1 axis expression.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of MAFG-AS1 as a detection reagent of a target site in the preparation of a drug for treating bladder cancer. Background technique [0002] Bladder urothelial carcinoma (BUC) is the most common malignant tumor in the urinary system. In recent years, the morbidity and mortality have increased year by year. There are about 430,000 newly diagnosed cases worldwide each year, resulting in 165,000 patient deaths each year. Bladder cancer is divided into non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC), of which NMIBC accounts for 70% to 80% of bladder cancer cases, and its main features are rapid progression and easy metastasis, nearly 75% of High-grade NMIBC recurs, progresses, or dies within 5 years of diagnosis; MIBC usually requires radical cystectomy, combined with neoadjuvant chemotherapy or radiotherapy, but eve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/158C12Q2600/118C12Q2600/112C12Q2600/178
Inventor 曹科肖梦卿向亮何东朱煜星曾庆海
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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