A kind of fungus effect factor and its coding gene and application
A technology of effectors and coding genes, applied in the field of molecular biology, can solve problems such as uncontrollable, economic loss, and crop failure
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Embodiment 1
[0066] 1. Experimental materials and methods
[0067] 1.1 Materials
[0068] 1.1.1 Test plants and strains
[0069] The tobacco used for the test was Nicotiana benthamiana preserved in our laboratory. Nicotiana benthamiana was planted in the greenhouse at a temperature of about 24°C and a humidity of about 70%, with 14 hours of light and 10 hours of darkness. The tobacco was grown for 25 days before being used for the test. The peppers tested are laboratory inbred varieties. The pepper seeds were placed in a large petri dish covered with absorbent paper and placed in a constant temperature incubator at 25°C for about 7 days. After the pepper seeds germinated, they were planted in In the flower pot with the substrate, the film is used to keep moisture. The post-emergence management was the same as that of Nicotiana benthamiana.
[0070] The test strains Phytophthora capsici LT1534 and SD33 were preserved in our laboratory; the Agrobacterium strains of Bax and INF1 were kindl...
Embodiment 2
[0173] In this example, the function of RxLR129113 was verified by silencing acetaldehyde dehydrogenase (NbALDH).
[0174] 1. Transient silencing of NbALDH gene in Nicotiana benthamiana
[0175] In order to verify whether ALDH affects the function of RxLR129113, the capsicum ALDH gene was compared with Nicotiana benthamiana genomic DNA, and the ALDH with the highest homology rate in Nicotiana benthamiana was NbALDH (nucleotide sequence shown in SEQ ID No.45).
[0176] From the N-terminal and C-terminal of NbALDH, two specific sequences NbALDH-1 (nucleotide sequence as shown in SEQ ID No.46) and NbALDH-2 (nucleotide sequence as shown in SEQ ID No.47) and NbALDH-2 (nucleotide sequence as shown in SEQ ID No.47) were screened out. shown), and constructed into the pTRV2 virus silencing vector, and after the sequence verification was correct, it was transferred to Agrobacterium GV3101.
[0177] 2. Verification of functional changes of RxLR129113 after silencing acetaldehyde dehydro...
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