A kind of aromatic ring compound and its preparation method and application
A compound and aromatic ring technology, applied in the field of aromatic ring compounds and their preparation, can solve problems such as research reports on secondary metabolites in non-petal sea mulberry fruit, and achieve good drug prospects, good alcohol affinity, and composition. safe effect
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Embodiment 1
[0024] Embodiment 1: Preparation of aromatic ring compound sonneradon A
[0025] The fruit of Sangria sinensis (wet weight about 50.0kg) was chopped, soaked and extracted three times with 60L of 95% industrial alcohol at room temperature, and soaked for one week each time to obtain a total of 110L of extracts, which were concentrated under reduced pressure (0.09MPa, 50°C ) to obtain the total extract 3.53kg of extract. The extract was extracted with n-butanol to obtain 6.1 L of n-butanol extract, and n-butanol was recovered under reduced pressure to obtain n-butanol extract (dry weight 346.48 g).
[0026] Dissolve 346.48 g of n-butanol extract in acetone, add appropriate amount of silica gel powder (200-300 mesh) and stir until uniform, dry at 50°C and grind to powder. Wet method was used to load the sample, and the normal phase silica gel column (V 有效 =5000cm 3 ) for separation. Choose to use CHCl 3 -MeOH system (CHCl 3 : MeOH=10:1, 5:1, 20:7, 0:10 / V:V) sequential gradi...
Embodiment 2
[0036] Anti-aging activity test of embodiment 2 compound sonneradon A
[0037] Culture medium and buffer preparation
[0038]
[0039]
[0040] Cultivation of nematodes: transfer the N2 wild-type adults from the old NGM plate to the new NGM plate on which OP50 Escherichia coli has been cultured to lay eggs, wash with 5mL M9 buffer after microscopic examination of a large number of eggs in the petri dish, and precipitate in the refrigerator at 4°C for 10 minutes. Aspirate part of the supernatant, the remaining 3.5ml, add 1.5ml of lysate, (use after mixing 1ml of 5M NaOH and 0.5ml of 5% Naclo) upside down for about 5min, centrifuge at 3000r / min for 1min, pour off the supernatant After adding 1ml of M9 buffer solution, 100 μL / plate was added to the culture dish and cultured in a 20°C incubator for 48h until the nematode L4 stage.
[0041] Preparation of OP50 E.coli fermentation broth: Inoculate a single colony of OP50 E.coli into LB liquid medium, culture overnight in a sh...
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