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Method for extracting novel triterpenoid Gvterpennoid A from Gendarussa vulgaris and application of Gvterpennoid A

The technology of a kind of compound, baloneol, which is applied in the field of extracting the new triterpenoid compound baloneol A, can solve the problem of low toxicity and side effects, and achieve the effects of easy preparation method, abundant raw materials, and huge economic and social benefits

Active Publication Date: 2020-05-22
HENAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The application of Chinese herbal medicine in anti-tumor has a long history. Finding high-efficiency and low-toxic anti-tumor active substances from Chinese herbal medicine and developing new anti-tumor drugs with strong selectivity and low toxicity and side effects are the most urgent problems for pharmaceutical researchers to solve, but so far There is no public report on how to extract the new triterpene compound Borchillene A from the small bone and use it to treat malignant tumors

Method used

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  • Method for extracting novel triterpenoid Gvterpennoid A from Gendarussa vulgaris and application of Gvterpennoid A
  • Method for extracting novel triterpenoid Gvterpennoid A from Gendarussa vulgaris and application of Gvterpennoid A
  • Method for extracting novel triterpenoid Gvterpennoid A from Gendarussa vulgaris and application of Gvterpennoid A

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Embodiment 1

[0020] In the specific implementation of the present invention, a method for extracting the new triterpene compound sclerophyllin A from small bark bone, using 20 kg of small bark bone as raw material, heating and refluxing ethanol with 5 times the weight of the raw material and a volume concentration of 95% Extracted 3 times, the extraction temperature was 90°C, and the extraction time was 1 hour each time, and the ethanol was recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract was suspended in 4.8L of distilled water, followed by petroleum Ether and ethyl acetate were extracted three times, 4.8L each time, for 1.5 hours; the ethyl acetate extraction part was separated by silica gel adsorption column chromatography, and the volume ratio was 100:5, 100:20, 100:30 petroleum ether in sequence - Acetone mixed solvent system for gradient elution, each gradient uses 5.0L eluent, the flow rate is 15mLmin -1 , collect 100:20 p...

Embodiment 2

[0022] The present invention is a method for extracting the new triterpene compound Bacterene A from small bark bone, using 15 kg of small bark bone as raw material, heating and refluxing 3 times with ethanol that is 3 times the weight of the raw material and having a volume concentration of 90%, and extracting The temperature is 95°C, the extraction time is 1 hour each time, and the ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract is suspended in 3.6L of distilled water, and the extract is mixed with petroleum ether and ethyl acetate in turn. Extract 3 times, 3.6L each time, and the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel adsorption column chromatography, and the volume ratio is 100︰5, 100︰20, 100︰30 petroleum ether-acetone mixed solvent system Carry out gradient elution, each gradient uses 3.7L eluent, the flow rate is 10mLmin -1 , collect 100:20 petroleum ether-ac...

Embodiment 3

[0024] The present invention is a method for extracting new triterpene compound Bacterene A from small bark bone, using 18 kg of small bark bone as raw material, heating and refluxing 3 times with ethanol that is 4 times the weight of the raw material and having a volume concentration of 85%, and extracting The temperature is 92°C, the extraction time is 1 hour each time, and the ethanol is recovered under reduced pressure to obtain the extract-like ethanol extract; the extract-like ethanol extract is suspended in 4.5L of distilled water, and the extract is mixed with petroleum ether and ethyl acetate in sequence. Extract 3 times, 4.5L each time, and the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel adsorption column chromatography, and the volume ratio is 100︰5, 100︰20, 100︰30 petroleum ether-acetone mixed solvent system Carry out gradient elution, each gradient uses 4.4L eluent, the flow rate is 12mLmin -1 , collect 100:20 petroleum ether-ac...

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Abstract

The invention relates to a method for extracting a novel triterpenoid, namely Gvterpennoid A from Gendarussa vulgaris and application of Gvterpennoid A. The method effectively solves problems in extracting the novel triterpenoid Gvterpennoid A from Gendarussa vulgaris for preparing anti-liver malignant tumor medicines. The method comprises the following steps: subjecting Gendarussa vulgaris to heating, refluxing and extracting with ethanol; recovering the ethanol under reduced pressure; suspending the obtained extractum-like ethanol extract in distilled water; sequentially carrying out extracting with petroleum ether and ethyl acetate three times; carrying out silica gel adsorption column chromatographic separation on an ethyl acetate extracted part; performing gradient elution by using apetroleum ether-acetone mixed solvent system; collecting a fraction, and recovering the solvent under reduced pressure to obtain a concentrate; carrying out reversed-phase ODS column chromatography; carrying out gradient elution by using a methanol-water mixed solvent system; collecting a fraction, and carrying out reduced-pressure concentration; carrying out silica gel column chromatography; andthen carrying out gradient elution by using a petroleum ether-acetone mixed solvent system, and collecting a fraction containing the target compoundto obtain Gvterpennoid A. According to the invention, used raw materials are rich in source, the preparation method is easy to operate, and the medicinal value and commercial value of Gendarussa vulgaris are increased.

Description

technical field [0001] The invention relates to medicine, in particular to a method for extracting a new triterpenoid compound bastilene A from a small bark bone and its application. Background technique [0002] Vulgaris vulgaris is the dry aerial part of Gendarussa vulgaris Nees. Widely distributed in my country's Guangxi, Guangdong, Hainan and other provinces. It has the effects of dispelling blood stasis and analgesia, reconnecting tendons and bones, and is mainly used for bruises, tendon injuries and fractures, rheumatic bone pain, blood stasis, amenorrhea and postpartum abdominal pain. It has a long history of application in our country, and it is recorded in ancient medical books such as "Supplements to Compendium of Materia Medica", "Preparation for the Properties of Herbs", "Lingnan Herbal Collection", and "Luchuan Materia Medica". In 2010, it was included in the Pharmacopoeia of the People's Republic of China as a new medicinal material. In recent years, the res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J7/00A61K31/57A61P35/00A61P1/16
CPCC07J7/007A61P35/00A61P1/16C07B2200/07
Inventor 孙彦君冯卫生陈辉张艳丽时博韩红艳李汉伟高美玲陈豪杰韩瑞杰赵晨
Owner HENAN UNIV OF CHINESE MEDICINE
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