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Application of rolipram in preparation of medicines for treating sepsis cardiac dysfunction

A technology of rolipram and sepsis, applied in the field of application of rolipram in the preparation of drugs for treating sepsis heart dysfunction

Inactive Publication Date: 2020-06-05
GENERAL HOSPITAL OF SOUTHERN THEATRE COMMAND OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of rolipram in the treatment of septic cardiac dysfunction.

Method used

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  • Application of rolipram in preparation of medicines for treating sepsis cardiac dysfunction
  • Application of rolipram in preparation of medicines for treating sepsis cardiac dysfunction
  • Application of rolipram in preparation of medicines for treating sepsis cardiac dysfunction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The experimental basis and animal level verification of rolipram in the treatment of sepsis cardiac function, this protocol also provides an experimental basis for the effect of rolipram on the in vitro cultured cardiac fibroblasts and endotoxemia models.

[0013] Animals and Reagents:

[0014] C57BL / 6 mice were purchased from Southern Medical University. All animal handling procedures have been approved by the Animal Care and Use Committee of Southern Medical University (permit number 2017J019), and all animal experiments were performed in accordance with the Animal Care Guidelines of Southern Medical University. LPS-lipopolysaccharide (L2630) and rolipram-rolipram (R6520) were purchased from Sigma-Aldrich. The enzyme Liberase TH purified research grade (054011501 ) used to digest cardiac tissue was purchased from Roche (Mannheim, Germany). RNA extraction reagent TRIzol was purchased from Life Technologies (Shanghai, China). Reverse transcription kit (FSQ-101) and R...

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PUM

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Abstract

The present invention discloses an application of rolipram in preparation of medicines for treating sepsis cardiac dysfunction. The rolipram can significantly reduce transcription and secretion of inflammatory factors in myocardial fibroblasts induced by lipopolysaccharide and up-regulate transcription and expression of bispecific phosphatase; a regulation function of the rolipram in regulating inflammation of the myocardial fibroblasts depends on the bispecific phosphatase; the rolipram can significantly reduce transcription levels and protein concentration of the inflammatory factors in endotoxemia heart tissues and up-regulate the transcription and expression of the bispecific phosphatase; the rolipram can significantly improve inflammatory cell infiltration degree of heart tissues of endotoxemia mice; and the rolipram can significantly improve a cardiac contractile function of the endotoxemia mice. The technical solution takes the myocardial fibroblasts as an intervention target, confirming that the rolipram can inhibit the myocardial fibroblast inflammation by up-regulating the bispecific phosphatase and significantly improve sepsis cardiac function.

Description

technical field [0001] The invention relates to the application of rolipram in the preparation of medicine for treating sepsis and cardiac dysfunction. Background technique [0002] Sepsis is an organ dysfunction caused by an immune imbalance caused by infection, and it is one of the leading causes of death in critically ill patients. Among them, about 40%-50% of patients with sepsis are complicated with cardiac dysfunction, and the risk of death of patients complicated with cardiac dysfunction is significantly increased. However, there is currently no recognized effective treatment for sepsis with cardiac dysfunction. Cardiac fibroblasts are important supporting cells in the heart tissue, and at the same time play an important role in immune surveillance. Under conditions such as infection and injury, they can be significantly activated, release inflammatory factors, etc., and amplify the inflammatory response. Studies in recent years have found that inflammatory factors m...

Claims

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Application Information

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IPC IPC(8): A61K31/4015A61P31/04A61P9/00A61P29/00
CPCA61K31/4015A61P9/00A61P29/00A61P31/04
Inventor 刘志锋吉晶晶苏磊
Owner GENERAL HOSPITAL OF SOUTHERN THEATRE COMMAND OF PLA
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