A preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin

A technology of albumin nanoparticles and baicalin, which is applied in the field of biomedicine, can solve the problems of low bioavailability of baicalin and the lack of active targeting performance of breast cancer treatment drugs, so as to improve tumor targeting and enhance anti-tumor Drug efficacy, non-antigenic effect

Active Publication Date: 2022-02-18
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing baicalin with low bioavailability and the existing breast cancer treatment drugs do not have active targeting performance, and provide a folic acid-coupled albumin nanoparticle loaded with baicalin The preparation method and application of

Method used

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  • A preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin
  • A preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin
  • A preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin

Examples

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Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Optimization of preparation process conditions for folic acid-coupled albumin nanoparticles (FA-BSANPs / BA) loaded with baicalin

[0058] 1. Optimization of the pH value of the buffer solution

[0059] (1) Experimental method

[0060] When the other preparation factors were fixed, the pH values ​​of the buffer solutions for dissolving folic acid-coupled albumin (FA-BSA) carriers were set to 7.0, 8.0, 9.0, and 10.0, respectively, to prepare FA-BSANPs / BA, and to measure the particle size diameter and encapsulation index (PDI).

[0061] (2) Experimental results

[0062] The effect of the pH value of the buffer solution on the particle size and encapsulation efficiency of FA-BSANPs / BA is shown in Table 1. It can be seen that with the increase of the pH value of the buffer solution, the particle size of FA-BSANPs / BA gradually decreases, but When the pH value of the buffer solution reaches 10.0, the PDI value increases; therefore, the pH value of the buffer soluti...

Embodiment 2

[0087] Example 2 Preparation of folic acid-coupled albumin nanoparticles loaded with baicalin

[0088] A preparation method of folic acid-coupled albumin nanoparticles loaded with baicalin, comprising the following steps:

[0089] Precisely weigh the prescribed amount of BA, dissolve it in an appropriate amount of absolute ethanol to obtain an absolute ethanol solution with BA dissolved; accurately weigh the prescribed amount of FA-BSA carrier, dissolve it in an appropriate amount of buffer solution with a pH value of 9.0, Configured as 1% concentration of FA-BSA carrier solution;

[0090] Under the conditions of 25°C and 500rad / min stirring, the absolute ethanol solution dissolved with BA was added to the FA-BSA carrier solution at a rate of 5mL / s, and the solution was placed under the ultrasonic probe (the transmission power of the ultrasonic probe was 300W) For 1 min, add 0.25% glutaraldehyde solution dropwise for cross-linking and solidification; finally transfer the solu...

Embodiment 3

[0092] Example 3 Preparation of folic acid-coupled albumin nanoparticles loaded with baicalin

[0093] A preparation method of folic acid-coupled albumin nanoparticles loaded with baicalin, comprising the following steps:

[0094] Precisely weigh the prescribed amount of BA, dissolve it in an appropriate amount of absolute ethanol to obtain an absolute ethanol solution with BA dissolved; accurately weigh the prescribed amount of FA-BSA carrier, dissolve it in an appropriate amount of buffer solution with a pH value of 7.0, Configured as 2% concentration of FA-BSA carrier solution;

[0095] Under the conditions of 24°C and 400rad / min stirring, add the absolute ethanol solution dissolved with BA into the FA-BSA carrier solution at a rate of 4mL / s, and place the solution under an ultrasonic probe for ultrasonication (the transmission power of the ultrasonic probe is 250W) 0.5min, dropwise add glutaraldehyde solution with a concentration of 1% for cross-linking and solidification...

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Abstract

The invention discloses a preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin. The invention provides a preparation method of folic acid-coupled albumin nanoparticles loaded with baicalin, comprising the following steps: S1. dissolving folic acid-coupled albumin carrier in a buffer solution to obtain a folic acid-coupled albumin carrier solution; S2. Add the dehydrated ethanol solution dissolved with baicalin into the carrier solution obtained in step S1 while stirring, ultrasonically, dropwise add glutaraldehyde solution for cross-linking and solidification, and spin evaporate the ethanol solution to obtain the baicalin entrapped Glycoside folic acid-coupled albumin nanoparticles. The preparation method of the nanoparticles is simple, the particle size is small, the dispersion is good, and the encapsulation efficiency is high, which overcomes the defect of poor water solubility of baicalin, effectively improves the bioavailability of baicalin, and improves the tumor targeting of nanoparticles It has the advantages of sex and antitumor efficacy, no toxic and side effects, and has broad application prospects as or in the preparation of drugs for treating breast cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to a preparation method and application of folic acid-coupled albumin nanoparticles loaded with baicalin. Background technique [0002] In recent years, data have shown that breast cancer has become one of the most common tumors in women in my country, accounting for about a quarter of malignant tumors in women. Worldwide, there are about 1.7 million new cases and about 410,000 deaths each year. There are 169,000 female breast cancer cases in my country every year, which is the second most common malignant tumor in women; about 45,000 Chinese women die from breast cancer, which is the sixth most common cause of malignant tumor death in women. At present, surgery is the main way to treat breast cancer, but it has the disadvantages of high cost, large trauma area, and high recurrence rate. Therefore, it is of great significance to develop safe and effective anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K47/42A61K47/22A61K31/7048A61P35/00
CPCA61K9/5052A61K9/5015A61K31/7048A61P35/00
Inventor 蔡宇马倩倩庄泳邹腾腾蓝萌李丽红杜曼玲
Owner JINAN UNIVERSITY
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