Device and method for uniformly mixing and enriching magnetic beads in anti-drug antibody detection

Abstract

The invention discloses a device and a method for uniformly mixing and enriching magnetic beads in anti-drug antibody detection, which can solve problem that the prior art is unreasonable, difficult uniform mixing and enrichment of magnetic beads, complex operation, time and labor waste and large difference of pretreatment data results of high-density enriched magnetic beads in pretreatment of a to-be-detected sample exist in the prior art. The device comprises a transverse platform, a motor and a vertical guide rail bracket; the vertical guide rail support is sequentially and fixedly connected with a magnetic bar fixing frame, a bar sleeve fixing frame and a cam movement sliding block device through sliding block devices from top to bottom in a sliding mode. A magnetic bar and a bar sleeve which are matched with each other are vertically, downwards and fixedly connected to the magnetic bar fixing frame and the bar sleeve fixing frame respectively, an opening is formed in the top of the bar sleeve, and the magnetic bar can be inserted into the opening of the bar sleeve; a rotating shaft of the motor drives the cam motion sliding block device to reciprocate up and down along the vertical guide rail bracket through the transmission device; meanwhile, the invention further provides a method which can be widely applied to the field of magnetic particle chemiluminescence immunodiagnosis.

Description

technical field [0001] The invention belongs to the field of magnetic particle chemiluminescence immunodiagnosis, and in particular relates to a mixing and enriching device and method for magnetic beads used in anti-drug antibody detection. Background technique [0002] In the anti-drug antibody detection method, high-density enrichment magnetic beads are used to pretreat the sample to be tested, which can detect the total ADA in the sample including the drug-ADA complex, and overcome the influence of high concentration of drug protein in the sample , Improve the drug resistance of ADA detection method. [0003] In the prior art, in the process of mixing and enriching magnetic beads used in the detection of anti-drug antibodies, it is necessary to face many inconvenient operations such as magnetic bead mixing, magnetic bead enrichment and separation for each sample, because most of them require manual Manual operation not only leads to complex processing steps, time-consumi...

AI Technical Summary

Problems solved by technology

[0007] (1) In step 2 and step 4, it is mentioned that the centrifuge tube is placed on the rotator for rotating and mixing, the mixing effect is poor, and because it is mixed upside down, it is easy to generate air bubbles in the process, which affects the treatment effect;

[0008] (2) Since each centrifuge tube can only process one sample, generally hundreds of samples need to be processed in one experiment, resulting in cumbersome processing of the existing device method, confusion in the transfer of centrifuge tubes, and poor operability;

[0009] (3) In steps 3 and 4, permanent magnets are used to adsorb the magnetic beads to one side of the centrifuge tube to separate the magnetic beads and the clarified solution in the solution. The separation process takes a long time and the adsorption rate of the magnetic beads is unstable. , leading to different pretreatments of the same sample, different effects, and large deviations in test results

[0010] (4) In the whole pretreatment process, the degree of manual participation is high, the process is time-consuming and complicated, and it is not convenient for the pretreatment of a large number of samples

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