Magnetic bead mixed liquid split charging device and method for nucleic acid substance extraction
A mixed liquid and magnetic bead technology, which is applied in the direction of mixing methods, mixers with rotating stirring devices, mixers, etc., can solve the problems of easy sedimentation of magnetic beads, uneven distribution of magnetic beads and microspheres, and achieve automatic mixing , performance guarantee, evenly distributed effect
Active Publication Date: 2021-06-15
XIAN TIANLONG SCI & TECH
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Problems solved by technology
[0008] The purpose of the present invention is to provide a magnetic bead mixed liquid dispensing device and a dispensing method for the extraction of nucleic acid substances in order to solve the problem of irreversible loss of adsorption function of the existing magnetic bead mixed liquid under low temperature conditions. At the same time, it solves the problem that the existing magnetic beads are easy to settle during the packaging process, resulting in uneven distribution of magnetic beads
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0048] The formula and preparation method of the protective solution are:
[0049] Take 0.1mol NaCl, 5% (V / V) AEO, 1% (V / V) absolute ethanol, stir at room temperature until fully dissolved, and then adjust the pH to 6 with Tris buffer.
Embodiment 2
[0051] The formula and preparation method of the protective solution are:
[0052] Take 2 mol of KCl, 15% (V / V) TritonX-100, 5% (V / V) isopropanol, stir at room temperature until fully dissolved, and then adjust the pH to 6 with Tris buffer.
Embodiment 3
[0054] The formula and preparation method of the protective solution are:
[0055] Take 5 mol of LiCl, 50% (V / V) PEG6000, and 10% (V / V) glycerol, stir at room temperature until fully dissolved, and then use Tris buffer to adjust the pH to 6.
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The invention discloses a magnetic bead mixed liquid split charging device and method for nucleic acid substance extraction. The device comprises a shell, a stirring device is arranged in the shell, the stirring device comprises a stirring part for stirring, the stirring part is arranged at the middle lower part of an inner cavity of the shell, the stirring part is arranged in the shell, the upper end of the stirring part is fixedly connected with the lower end of a suction pipe of a tubular structure, the upper end of the suction pipe is communicated with a negative pressure device through a conveying pipe, a rotating device used for providing rotating force power is arranged on the suction pipe, the negative pressure device and the rotating device are arranged outside the shell, the rotating device drives the stirring part to rotate through the suction pipe, and the suction pipe is used for discharging the magnetic bead mixed liquid in the shell. By means of the split charging device and the split charging method, automatic even mixing and automatic split charging of the magnetic bead mixed liquid can be achieved, the adsorption performance of the magnetic beads can be guaranteed under the low-temperature condition, time and labor are saved, operation is standard, cost is low, and the device and the method are particularly suitable for industrial production.
Description
technical field [0001] The invention relates to the technical field of magnetic bead mixed liquid production, in particular to a magnetic bead mixed liquid dispensing device and a dispensing method for nucleic acid substance extraction. Background technique [0002] With the development of the times, the application of molecular biological detection methods is becoming more and more extensive, and the corresponding nucleic acid extraction and purification are also paid more and more attention and attention. Magnetic bead nucleic acid extraction technology is because of its simplicity, rapidity and easy implementation. Automation was rapidly promoted. The core of this method is the magnetic bead, which is a spherical biological nanomaterial formed by coating silicon dioxide on the outside of ferric oxide. The particle size is usually tens of nanometers to hundreds of nanometers. The surface of the magnetic bead With hydroxyl functional groups, under the conditions of high co...
Claims
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IPC IPC(8): B01F7/16B01F7/18B01F3/08B01F15/02
CPCB01F23/43B01F27/192B01F27/80B01F27/90B01F35/75465
Inventor 李红东卜振中孙瑶
Owner XIAN TIANLONG SCI & TECH
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