A kind of rice composite fermentation product and its preparation method and application
A technology for compound fermentation and rice, applied in the field of fermentation to achieve good tyrosinase activity and inhibit tyrosinase activity
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Embodiment 1
[0060] Example 1 Preparation of rice composite fermentation puree
[0061] 1. Activation of strains: Pick Lactobacillus delbruechiisubsp. Bulgaricus colonies in two loops and inoculate them into a test tube of 100 mL MRS liquid medium, and activate at 35°C for 24 hours.
[0062] 2. Purification of bacterial strains: After gradient dilution of the above-mentioned liquid bacterial strains, 200 μL was inoculated and spread on MRS solid medium plate, and cultured at 35° C. for 48 hours.
[0063] 3. Liquid culture: Take 3 rings of single colonies from the above plate, inoculate them in 100 mL of MRS liquid medium, and activate at 35°C for 48 hours.
[0064] 4. Expanded culture: Take the above liquid test tube with 10% of the inoculum, insert it into 100 mL MRS liquid medium, and cultivate at 37°C for 12 hours. When the bacterial volume reaches 10 8 At CFU / mL, seed liquid was obtained.
[0065] 5. Preparation of fermentation substrate: take 0.05 g of pomegranate fruit extract (10:...
Embodiment 2
[0069] Example 2 Application of Rice Compound Fermented Puree as Cosmetics
[0070] 1. Safety testing of rice compound fermented puree
[0071] Human patch test is mainly used to detect the irritation of final cosmetic products or raw materials. According to the "Hygienic Standard for Cosmetics" (2015), the present disclosure conducts a human closed patch test on the rice composite fermented puree obtained in Example 1, in order to evaluate its potential skin irritation.
[0072] 1. Test object:
[0073] 30 suitable volunteers were selected for this trial, and the age range was 18-60 years old.
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