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Method for evaluating fitness of hybridization or backcrossing offspring of Glycine max and Glycine soja Sieb.et Zucc.

A technology of transgenic soybeans and wild soybeans, applied in plant genetic improvement, botanical equipment and methods, legume cultivation, etc., can solve problems such as invasion, changing the survival and reproductive ability of wild populations, and difficult to control weeds

Pending Publication Date: 2020-07-10
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When these transgenes are transferred to populations of wild relatives through gene drift, it may change the survival and reproduction capabilities of these wild populations, and even their ability to invade, causing them to rapidly expand their distribution space or form weeds that are difficult to control, resulting in unpredictability environmental impact

Method used

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  • Method for evaluating fitness of hybridization or backcrossing offspring of Glycine max and Glycine soja Sieb.et Zucc.
  • Method for evaluating fitness of hybridization or backcrossing offspring of Glycine max and Glycine soja Sieb.et Zucc.
  • Method for evaluating fitness of hybridization or backcrossing offspring of Glycine max and Glycine soja Sieb.et Zucc.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Evaluation on the fitness of F2 generation of glyphosate-resistant transgenic soybean and BY-21 wild soybean

[0068] Experimental materials for establishing hybrids of transgenic soybeans and wild soybeans and their progeny:

[0069] On the basis of fully understanding the growth characteristics of transgenic soybeans and BY-21 wild soybeans, the transgenic soybeans and BY-21 wild soybeans were planted in batches so that the flowering stages of the two met; the transgenic soybeans and BY-21 wild soybeans were obtained by artificial hybridization The F1 generation of hybrids, and then the F2 generation test materials were obtained by selfing of F1.

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Abstract

The invention belongs to the field of ecological safety evaluation of Glycine max, and particularly relates to a method for evaluating the fitness of the hybridization or backcrossing offspring of Glycine max and Glycine soja Sieb.et Zucc.. The characters, which most favorably reflect viability and fertility of a hybridization or backcrossing offspring of the Glycine max and Glycine soja Sieb.et Zucc. in a whole life history period, can be calculated and measured, and finally, total fitness can be used for clearly obtaining the fitness of the hybridization or backcrossing offspring of the Glycine max and the Glycine soja Sieb.et Zucc., wherein the characters include four characters of a vegetative period and eight characters of a reproductive period. According to the method disclosed by the invention, the problem that no methods for evaluating the fitness of the hybridization or backcrossing offspring of the Glycine max and the Glycine soja Sieb.et Zucc. are provided are present can besolved. The method has an important function for scientifically evaluating the fitness of the hybridization or backcrossing offspring of the Glycine max and the Glycine soja Sieb.et Zucc.. The evaluation of the fitness is an important link for safely releasing the Glycine max, and after the Glycine max is subjected to gene flow to the Glycine soja Sieb.et Zucc., gene flow prevention work is carried out on an influence on the fitness of the Glycine soja Sieb.et Zucc. in advance.

Description

technical field [0001] The invention relates to a method for evaluating the fitness of hybrid or backcross progeny of transgenic soybeans and wild soybeans, and belongs to the field of ecological safety evaluation of transgenic soybeans. Background technique [0002] Since the commercial planting of the world's first genetically modified crop (transgenic storage-resistant tomato) in 1996, the research and application of genetically modified biotechnology and genetically modified products have made great progress. On December 30, 2019, the Department of Science, Technology and Education of the Ministry of Agriculture and Rural Affairs formulated Approved to issue agricultural genetically modified biological safety certificate catalog, which includes glyphosate-resistant genetically modified soybean variety SHZD32-01. The rapid development of genetically modified biotechnology and the global commercial application of genetically modified crops provide a new option for increasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G22/40
CPCA01H1/02A01G22/40
Inventor 宋小玲强胜盛泽文刘金悦胡玉琪刘琪
Owner NANJING AGRICULTURAL UNIVERSITY
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