Method for evaluating fish intestinal tract tissue slice

Abstract

The invention discloses a method for evaluating a fish intestinal tract tissue slice, and belongs to the field of aquatic products. The method comprises the following steps: observing a mucous membrane layer, a submucous membrane layer, a muscular layer and a serosa layer of a target tissue slice by using a microscope, and respectively scoring observed symptom results; wherein the weights of the mucous membrane layer, the submucous membrane layer, the muscular layer and the serous membrane layer are b1, b2, b3 and b4 respectively, b1 is larger than b2, b3 is larger than b4, and b1 + b2 + b3 +b4 = 1; score of intestinal tissue slices. According to the invention, a weighted scoring method is adopted, an intestinal tissue pathological symptom index scoring standard is established, comprehensive quantitative evaluation is carried out on the intestinal tract tissue slices. The weight of different symptoms of the same structural layer and the weight of different structural layers of intestinal tissues are determined according to the importance degree, finally, the grade score of each symptom is multiplied by the symptom weight to obtain the score of each structural layer, all symptoms of each intestinal slice are comprehensively considered and finally converted into data for statistical analysis, and the data is objective and comprehensive.

Description

technical field [0001] The invention relates to the field of aquatic products, in particular to an evaluation method for fish intestinal tissue slices. Background technique [0002] The currently commonly used evaluation method for intestinal slices is to use the description or measurement of some local parts of an individual intestinal slice to represent the level of the treatment group. However, the differences in intestinal tissue slices are relatively large, mainly in: the intestinal slices of different fish in the same treatment group are different, the lesion degree of different tissue structures in the same intestinal ring is different, and the degree of lesion in the same intestinal ring is different. There are differences between different views of the same organizational structure. Secondly, there are not many quantifiable indicators for intestinal slices, and the measurement results are easily affected by the quality of the slices and the subjectivity of the obse...

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Problems solved by technology

[0003] In order to overcome the problems of one-sided evaluation results, large differences, and low accuracy of evaluation structures in the prior art, the present invention provides an evaluation method for fish intestinal tissue slices, forming a relatively systematic, comprehensive and complete set of evaluation methods. The evaluation method improves the systematicness and accuracy of the evaluation of fish intestinal tissue slices

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