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Single-cell hemoglobin determination method and device based on image processing

A hemoglobin and image processing technology, applied in the field of image processing, can solve the problems of complex determination process, inability to accurately measure the area of ​​a single red blood cell and hemoglobin content, etc., and achieve the effect of accurate calculation

Pending Publication Date: 2020-07-24
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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Problems solved by technology

[0003] There are at least the following technical problems in the prior art: the use of biochemical methods to measure single-cell hemoglobin content requires biochemical laboratory conditions, the measurement process is complicated, and the use of biochemical methods cannot accurately measure the area of ​​​​a single red blood cell and the content of hemoglobin

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  • Single-cell hemoglobin determination method and device based on image processing
  • Single-cell hemoglobin determination method and device based on image processing
  • Single-cell hemoglobin determination method and device based on image processing

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Embodiment Construction

[0043] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in combination with specific embodiments and with reference to the accompanying drawings. It should be understood that these descriptions are exemplary only, and are not intended to limit the scope of the present invention. Also, in the following description, descriptions of well-known structures and techniques are omitted to avoid unnecessarily obscuring the concept of the present invention.

[0044] Such as figure 1 As shown, one aspect of the present invention provides a single-cell hemoglobin assay method based on image processing, the method comprising:

[0045] S11. Convert the RGB image of the blood sample slide into an HSV image of the blood sample slide, extract the V-channel image of the blood sample slide, and perform binarization processing on the V-channel image of the blood sample slide according ...

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Abstract

The invention discloses a single-cell hemoglobin determination method and device based on image processing. The method comprises the following steps of: converting a blood sample slide RGB image intoa blood sample slide HSV image, extracting a blood sample slide V channel image, and performing binarization processing on the blood sample slide V channel image according to a set first threshold value to obtain a blood sample slide binarization image; carrying out cell image segmentation processing on the blood sample slide binarization image to extract a single erythrocyte image, and carrying out binarization processing on the single erythrocyte image according to a set second threshold value to remove a central lightly-stained area of erythrocytes; graying the single erythrocyte image of the erythrocyte non-central lightly-stained area to obtain a single erythrocyte gray level image, and calculating the area and hemoglobin content of the whole erythrocyte according to the single erythrocyte gray level image. The method can be adopted to accurately calculate the area of a single erythrocyte and the hemoglobin content.

Description

technical field [0001] The invention relates to the technical field of image processing, in particular to a method and device for measuring single-cell hemoglobin based on image processing. Background technique [0002] Hemoglobin content is a carrier protein responsible for carrying oxygen and carbon dioxide in higher organisms (abbreviated as Hb or HGB). Hemoglobin is the protein that makes blood red. The content of hemoglobin in the human body is a reliable indicator for determining anemia. At present, the single-cell hemoglobin content is usually measured by biochemical methods, specifically, the cell membrane is broken to release hemoglobin, and then the single-cell hemoglobin content is obtained by measuring with chemical agents. [0003] There are at least the following technical problems in the prior art: the use of biochemical methods to measure single-cell hemoglobin content requires biochemical laboratory conditions, the measurement process is complicated, and the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T7/62G06T7/11G06T7/136G06T7/90G01N21/84
CPCG06T7/0012G06T7/62G06T7/11G06T7/136G06T7/90G01N21/84G06T2207/10056G06T2207/30004
Inventor 吴卫王庚王欣陈倩李柏蕤刘丹
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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