Adaptive Aberration Correction Method and Light Sheet Microscopic Imaging Device Based on the Method
An aberration correction and microscopic imaging technology, applied in the field of optical imaging, can solve the problems of slow aberration correction speed, small aberration range, limited imaging depth, etc., and achieve fast aberration correction speed and high aberration correction accuracy. , the effect of simple operation
Active Publication Date: 2022-02-18
哈工大机器人(中山)无人装备与人工智能研究院
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Problems solved by technology
[0002] Light sheet microscopy has the advantages of low bleaching of biological tissues and high axial resolution, but its imaging depth is limited. The development of research in biomedical and other fields has put forward higher requirements for the imaging depth of modern microscope systems
As the imaging depth increases, the aberrations introduced by the refractive index mismatch will seriously affect the quality of microscopic imaging. This type of aberration will change with the observation environment and the sample itself, so it is necessary to introduce adaptive optics technology Perform real-time dynamic correction of aberrations
[0003] The range of aberrations corrected by the existing adaptive optics technology is too small, and the aberration correction speed is too slow
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[0043] In order to make the above objects, features and advantages of the present invention more comprehensible, specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0044] In order to facilitate the understanding of the present invention, a brief introduction to the adaptive optics technology is given first.
[0045] Adaptive optics technology mainly includes direct wavefront detection technology that relies on wavefront detectors and indirect wavefront detection technology that does not rely on wavefront sensors. Various embodiments of the present invention are applicable to the indirect wavefront detection technology that does not rely on the wavefront sensor, and the adaptive system without the wavefront sensor includes a wavefront controller and a wavefront corrector, wherein the wavefront controller is used to control the wavefront according to the wavefront control algorithm , calculate the cont...
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The invention provides an adaptive aberration correction method and a light sheet microscopic imaging device based on the adaptive aberration correction method. The adaptive aberration correction method includes: establishing a Zernike polynomial representation model of aberration; A genetic algorithm is used to solve the approximate optimal solution of the Zernike polynomial representation model; taking the approximate optimal solution as a starting point, a stochastic parallel gradient descent algorithm is used to further solve the global optimal solution of the Zernike polynomial representation model; based on the The global optimal solution calculates the phase to be corrected, where the phase to be corrected is an aberration correction parameter. The adaptive aberration correction method of the present invention can correct a wide range of aberrations and has a high aberration correction speed.
Description
technical field [0001] The invention relates to the technical field of optical imaging, in particular to an adaptive aberration correction method and a light sheet microscopic imaging device based on the adaptive aberration correction method. Background technique [0002] Light sheet microscopy has the advantages of low bleaching of biological tissues and high axial resolution, but its imaging depth is limited. The development of research in biomedical and other fields has put forward higher requirements for the imaging depth of modern microscopy systems. As the imaging depth increases, the aberrations introduced by the refractive index mismatch will seriously affect the quality of microscopic imaging. This type of aberration will change with the observation environment and the sample itself, so it is necessary to introduce adaptive optics technology Perform real-time dynamic correction of aberrations. [0003] The aberration range corrected by the existing adaptive optics ...
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IPC IPC(8): G02B27/00G01N21/64
CPCG02B27/0025G02B27/0012G01N21/6458
Inventor王伟波李晓君詹天鹏谭久彬
Owner哈工大机器人(中山)无人装备与人工智能研究院