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Application of Gankyrin protein as novel molecular marker in prognosis evaluation of prostate cancer

A Prostate Cancer, Prognosis Technology, Applied in Biological Field

Pending Publication Date: 2020-08-28
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the relationship between Gankyrin and prostate cancer progression and resistance to antiandrogen therapy, and the relationship between Gankyrin and tumor-associated macrophages in the prostate cancer microenvironment have not been studied. There is no literature report on biomarkers to assess treatment prognosis and guide individualized treatment

Method used

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  • Application of Gankyrin protein as novel molecular marker in prognosis evaluation of prostate cancer
  • Application of Gankyrin protein as novel molecular marker in prognosis evaluation of prostate cancer
  • Application of Gankyrin protein as novel molecular marker in prognosis evaluation of prostate cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The application of the new molecular marker prostate cancer prognosis assessment model includes the following technical steps:

[0055] 1. Acquisition of prostate cancer biopsy samples for immunohistochemical staining

[0056] A. Paraffin sections were baked at 65°C for 1 hour, then dewaxed and hydrated: soak in xylene three times, 8 minutes / time, soak in 100%-75% gradient alcohol four times, 8 minutes / time, and finally place in phosphate buffer (PBS).

[0057] B. Use high temperature to carry out corresponding antigen restoration, cool naturally at room temperature, circle the tissue area with an immunohistochemical pen, cover with 3% hydrogen peroxide for 20 minutes, wash with PBS 3 times for 5 minutes each time, and block with goat serum for 1 hour at room temperature.

[0058] C. Wash once with PBS, add Gankyrin or CD68 primary antibody, overnight at 4°C.

[0059] D. The next day, wash 3 times with PBS, and incubate with secondary antibody (generic for pika, bioti...

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Abstract

The invention relates to the technical field of biology, in particular to a prognosis evaluation method of a Gankyrin protein and a tumor-associated macrophage marker CD68 combined with the Gankyrin protein as a novel molecular marker in a prostate cancer pathological specimen. According to the invention, the capability of Gankyrin as a prognostic typing molecular marker in prostate cancer is verified; gankyrin and CD68 immunohistochemical staining is carried out on a prostate biopsy pathological specimen of a patient; after immunohistochemical scoring, high expression and low expression of Gankyrin and CD68 are distinguished according to corresponding truncation values; patients are divided into three groups, i.e., a high risk (high expression of Gankyrin and high expression of CD68), a medium risk (high expression of Gankyrin or high expression of CD68) and a low risk (low expression of Gankyrin and low expression of CD68), for drug resistance and transfer risk of anti-androgen therapy.

Description

technical field [0001] The invention relates to the field of biotechnology, specifically, the application of Gankyrin protein itself and Gankyrin protein combined with tumor-associated macrophage marker CD68 as a novel molecular marker in prognostic assessment of prostate cancer. Background technique [0002] Prostate cancer (PCa) is the most common tumor in males in developed countries. In recent years, the incidence of prostate cancer in domestic males has also increased year by year. Although the treatment effect of early prostate cancer is better, the survival rate of patients with metastasis and resistance to anti-androgen therapy will decrease significantly. If we can use the prostate biopsy performed when the patient is first diagnosed to identify patients with possible metastasis and resistance to anti-androgen therapy at the early stage of diagnosis and carry out personalized intervention, we can improve the prognosis of patients with advanced prostate cancer from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/57434G01N2800/52G01N2800/56
Inventor 高旭王超彭广董克勤瞿旻杨波张超
Owner SHANGHAI CHANGHAI HOSPITAL
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