Rubus parvifolius fractional polysaccharide, preparation method and application of rubus parvifolius fractional polysaccharide in depression resistance

A technology of polysaccharides and mulberry, which is applied in the field of polysaccharides, can solve the problems of extraction and separation of berry polysaccharides that have not been seen before, and achieve the effects of shortening the immobility time of the tail, high purity, and obvious activity

Active Publication Date: 2020-10-16
山东聚胜生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no extraction, separation and application of mooseberry polysaccharide in antidepressant treatment

Method used

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  • Rubus parvifolius fractional polysaccharide, preparation method and application of rubus parvifolius fractional polysaccharide in depression resistance
  • Rubus parvifolius fractional polysaccharide, preparation method and application of rubus parvifolius fractional polysaccharide in depression resistance
  • Rubus parvifolius fractional polysaccharide, preparation method and application of rubus parvifolius fractional polysaccharide in depression resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Collect the leaves of Rubus parvifolius L., wash and dry in the shade, and crush them for later use.

[0026] Take dried Maomei leaves and add absolute ethanol at a solid-liquid ratio (w / v) of 1:10, soak for 48 hours, filter, collect defatted Maomei leaves, and evaporate the solvent for later use.

[0027] Take 1kg of the above-mentioned degreased Maomei leaves, add 10L of distilled water, heat and extract at 85°C for 2 hours, collect the filtrate and filter residue by filtration, repeat the extraction of the filter residue once, combine the extracts obtained from the two extractions, concentrate under reduced pressure to 2L, and centrifuge to remove insoluble After adding 8L of ethanol and stirring evenly, the mixture was left to settle for 12 hours, and then centrifuged to collect the precipitate. The precipitate was redissolved with distilled water, dialyzed with distilled water in 8000-14000 Da dialysis bag for 48 hours, the liquid in the dialysis bag was collected,...

Embodiment 2

[0032] 1. Test materials

[0033] The graded polysaccharides of Maoberry were prepared according to the method in Example 1, and stored at 4°C for future use.

[0034] SPF grade male Kunming mice, body weight (20±2) g, were purchased from Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing. During the experiment, the mice had free access to water and food, and were adaptively fed for one week before the experiment.

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Abstract

The invention discloses rubus parvifolius fractional polysaccharide, a preparation method and an application of the rubus parvifolius fractional polysaccharide in depression resistance. The inventionprovides the fractionated polysaccharide which is extracted and separated from the rubus parvifolius leaves and has the alcohol solubility of 40%-50%, the polysaccharide content is (95.3+/-2.7)%, andthe purity is high; it is found that the rubus parvifolius fractional polysaccharide can remarkably shorten the tail suspension immobility time and the swimming immobility time in a mouse tail suspension test and a mouse forced swimming test, plays a role similar to that of a positive drug, and has obvious anti-depression activity. The rubus parvifolius polysaccharide provided by the invention isa natural active polysaccharide and has no toxic or side effect.

Description

technical field [0001] The invention relates to the field of polysaccharides, in particular to a graded polysaccharide from mulberries, a preparation method and an antidepressant application. Background technique [0002] Depression is an affective mental disorder caused by genetic, endocrine, metabolic, neurobiological, and environmental factors. The main clinical manifestations are depressed mood, lack of interest, and anhedonia. Depression has a high recurrence rate and suicide rate, and the World Health Organization pointed out that depression may become the second largest disease after coronary heart disease. At present, Western medicine treatment can relieve depression symptoms to a certain extent, but there are often problems such as high recurrence rate and large side effects. [0003] Natural active polysaccharides are natural macromolecules composed of several monosaccharides with α- or β-glycosidic bonds widely present in plants, animals and microorganisms. Becau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P25/24
CPCC08B37/0003C08B37/00A61K31/715A61P25/24
Inventor 于海涛其他发明人请求不公开姓名
Owner 山东聚胜生物科技有限公司
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