Three-dimensional reconstruction method of gold nanospheres based on polarization parameter microscopic sequence image
A technology of sequential images and gold nanospheres, applied in the field of 3D reconstruction, can solve the problems of large errors of gold nanosphere models, and achieve the effects of good surface continuity, high precision and small errors.
Active Publication Date: 2020-11-13
NANJING UNIV OF SCI & TECH
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[0003] The purpose of the present invention is to propose a three-dimensional reconstruction method of gold nanospheres based on polarization parameter microscopic sequence images, to solve the problem that the gold nanosphere model reconstructed by traditional microscopic three-dimensional reconstruction methods has a large error with the original sample
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[0051] like Figure 7 As shown, a three-dimensional reconstruction method of gold nanospheres based on polarization parameter microscopic sequence images, the specific steps are:
[0052] S1, such as figure 1 As shown, the polarization parameter microscope scanning step is set to 10 nanometers, the sample is placed on the stage, and the control software on the computer is used to control the sample and the stage to move along the fixed step in the direction close to the eyepiece, and then in During the translation process, a polarization parameter microscope was used to scan the sample of gold nanospheres, and a total of 18 microscopic images were obtained successively. The pixel resolution of each microscopic image is 103×84, and the corresponding length and width of each pixel are 10 nanometers. ;
[0053] S2, such as figure 2 As shown in , the obtained tomographic sequence image is preprocessed to obtain the sequence image of the target area;
[0054] Specifically, the...
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The invention provides a three-dimensional reconstruction method of gold nanospheres based on a polarization parameter microscopic sequence image. The method comprises steps of scanning through a polarization parameter microscope to obtain a microscopic chromatography sequence image of the gold nanospheres; arranging the sequence images into a three-dimensional data field at equal intervals from bottom to top according to a time sequence; performing filtering, enhancing, registering, segmenting, extracting a target and the like on the tomographic sequence image to obtain a target contour of the intermediate layer image; determining a threshold value of each layer of image through sphere fitting and a broken line graph representing a relationship between a gray value and a pixel point coordinate, comparing the gray value of the pixel point with the threshold value of the layer of image to determine an internal point, connecting the internal point by using a non-uniform rational B splinecurve, and finally rendering to obtain a three-dimensional model of the gold nanosphere. The method has the advantages of being small in error, high in precision and good in surface continuity.
Description
technical field [0001] The invention belongs to the technical field of microscopic three-dimensional reconstruction, in particular to a method for three-dimensional reconstruction of gold nanospheres based on polarization parameter microscopic sequence images. Background technique [0002] Microscopic 3D reconstruction technology is an important research direction of computer vision, which is widely used in medical image processing and structural biology. Traditional 3D reconstruction algorithms for microscopic images mainly include: fault contour-based algorithms, moving cube algorithms, ray-casting algorithms, snowball-throwing algorithms, miscutting-deformation methods, and hardware-based 3D texture mapping methods. Gold nanospheres are widely used in various researches in the fields of life science and medicine. They have good biocompatibility and high fluorescence emission efficiency. They can be used as fluorescent contrast agents. Because nanoscale gold spheres With ...
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IPC IPC(8): G06T17/20G06T7/13G06T3/40G06T7/90G06F17/12
CPCG06T17/20G06T7/13G06T3/4023G06T7/90G06F17/12G06T2207/10061
Inventor叶星园熊吉川刘学峰顾凯徐彬刘卫平金霄
OwnerNANJING UNIV OF SCI & TECH