Droplet delay calculation device and calculation method thereof

Abstract

The invention relates to a droplet delay calculation device and a droplet delay calculation method. The droplet delay calculation device comprises at least one laser, a flow chamber, a nozzle, a firstdetection unit, a second laser, a second detection unit, an image acquisition unit, a charging device and a sorting plate; the first detection unit comprises a second detector, and the second detector is used for detecting a scattered light signal and acquiring the time when particles reach the detection point; the second laser is used for emitting at least one laser, the second detection unit isused for analyzing a second fluorescence signal, the second detection unit comprises a third detector, and the third detector is used for detecting a second fluorescence signal and obtaining the timewhen the particles reach a breaking point. By arranging the first detector, the second detector and the third detector, the droplet delay time can be directly measured, adjustment of the droplet delay time is avoided, the advantages of being short in consumed time and capable of saving consumables are achieved, and the sorting efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of cell sorting technology equipment, in particular to a device and method capable of automatically calculating droplet delay. Background technique [0002] With the development of social economy and the advancement of science and technology, liquid droplets are widely used in industry, medicine and life. By applying periodic disturbances to the liquid flow ejected from the nozzle, the liquid flow is gradually broken into droplets that wrap cells. When a beam of laser light is just ejected from the nozzle, the scattered light generated by a cell wrapped in it is projected onto the Photomultiplier tubes, which are converted into electrical signals, are identified. In this process, the time from when a cell is identified to when the droplet containing the cell leaves the flow is called the droplet delay. [0003] At present, the conventional devices for calculating droplet delay mainly include the following types: ...

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Problems solved by technology

[0009] However, the above-mentioned method of setting the droplet delay often requires multiple measurements to obtain an accurate droplet delay time, which takes a long time, is inconvenient to operate, and has low efficiency.

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