Droplet delay calculation device and calculation method

Abstract

The invention relates to a droplet delay calculation device and a calculation method thereof. The device of the invention includes at least one laser, a flow chamber, a nozzle, a first detection unit, a second laser, a second detection unit, an image acquisition unit, a charging device and a sorting device. plate; the first detection unit includes a second detector, the second detector is used to detect the scattered light signal, and is used to obtain the time when the particle reaches the detection point; the second laser is used to emit at least one kind of laser light, and the second detector The unit is used to analyze the second fluorescence signal, the second detection unit includes a third detector, the third detector is used to detect the second fluorescence signal, and is used to obtain the time when the particle reaches the breaking point; the present invention sets the first The detector, the second detector and the third detector can directly measure the delay time of the droplet and avoid adjusting the delay time of the droplet, which has the advantages of short time consumption and saving consumables, and greatly improves the sorting efficiency.

Description

technical field [0001] The invention relates to the field of cell sorting technology equipment, in particular to a device and method capable of automatically calculating droplet delay. Background technique [0002] With the development of social economy and the advancement of science and technology, liquid droplets are widely used in industry, medicine and life. By applying periodic disturbances to the liquid flow ejected from the nozzle, the liquid flow is gradually broken into droplets that wrap cells. When a beam of laser light is just ejected from the nozzle, the scattered light generated by a cell wrapped in it is projected onto the Photomultiplier tubes, which are converted into electrical signals, are identified. In this process, the time from when a cell is identified to when the droplet containing the cell leaves the flow is called the droplet delay. [0003] At present, the conventional devices for calculating droplet delay mainly include the following types: ...

AI Technical Summary

Problems solved by technology

[0009] However, the above-mentioned method of setting the droplet delay often requires multiple measurements to obtain an accurate droplet delay time, which takes a long time, is inconvenient to operate, and has low efficiency.

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