Application of dabrafenib and/or its pharmaceutically acceptable derivatives in the preparation of anti-arenavirus drugs
A virus and drug technology, applied in the field of medicine, can solve the problems of limited therapeutic effect and strong side effects of anti-arenavirus drugs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] This example studies the cytotoxicity of the target compound dabrafenib, and the specific process is as follows:
[0026] BHK-21 cells and Vero cells were divided into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, replace the medium with DMEM medium containing 2% FBS, and then use dabrafenib at a concentration of 0.5 μM, 1 μM, 2 μM, 4 μM, 8 μM, 16 μM, 32 μM, 64 μM and 128 μM For treatment, add the same volume of DMSO to the negative control, set three replicate wells for each concentration, incubate at 37°C for 36 hours, and set up zero-adjustment wells with only medium and no cells. Discard the supernatant, wash once with PBS, add 50 μl of 0.5% MTT PBS solution to each well, and place it at 37°C for 4 hours in the dark, and you can observe blue-purple crystals appearing on the bottom of the plate, and stop the culture at this time. And carefully aspirate the supernatant, add 100 μl DM...
Embodiment 2
[0029] This example studies the antiviral activity of the target compound dabrafenib against arenavirus (LCMV), the specific process is as follows:
[0030] BHK-21 cells and Vero cells were divided into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, replace the medium with DMEM medium containing 2% FBS, and then use dabrafenib at a concentration of 0.5 μM, 1 μM, 2 μM, 4 μM, 8 μM, 16 μM, 32 μM, 64 μM and 128 μM For treatment, the same volume of DMSO was added to the negative control, and three replicate wells were set for each concentration. After incubation for 1 h, cells were infected with LCMV. After 36h, the effect of dabrafenib inhibiting virus replication was detected by measuring the supernatant titer, and the results on the two kinds of cells were as follows: Figure 5 and Figure 6 As shown, under different concentration conditions, the compound dabrafenib has a significant inhibitor...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com