Digital nucleic acid amplification detection method and integrated detection system based on CRISPR and Cas
A detection system and detection method technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as aerosol pollution, poor integration, etc., to ensure accuracy and avoid crossover Pollution, the effect of improving the universality of use
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[0049] The present invention will be further described below in conjunction with the accompanying drawings, but the present invention is not limited to the following embodiments.
[0050] The specific implementation includes an integrated reaction chip 3, a temperature control module, a light source, and an optical signal detector; as image 3 As shown, the integrated reaction chip 3 is distributed with an amplification system droplet generation area 4, a nucleic acid amplification area 6, a detection system droplet generation area 7, a droplet fusion area 8, and an optical detection area 9; the amplification system droplet Between the generation area 4 and the nucleic acid amplification area 6, between the nucleic acid amplification area 6 and the droplet fusion area 8, between the detection system droplet generation area 7 and the droplet fusion area 8, and between the droplet fusion area 8 and the optical detection The zones 9 are all connected by microchannels 5 .
[0051...
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