Method for primary isolated culture of porcine mammary epithelial cells (PMEC)

A technique for separating and culturing mammary gland epithelial cells is applied in the field of primary isolation and culture of mammary gland epithelial cells of sows, and can solve the problems of less research and application of porcine mammary glands.

Inactive Publication Date: 2020-12-08
陈浩东 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While most of the researches are focused on bovine mammary glands and relatively mature, there are few researches and applications on porcine mammary glands

Method used

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  • Method for primary isolated culture of porcine mammary epithelial cells (PMEC)
  • Method for primary isolated culture of porcine mammary epithelial cells (PMEC)
  • Method for primary isolated culture of porcine mammary epithelial cells (PMEC)

Examples

Experimental program
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Embodiment 1

[0024] The mammary glands of non-pregnant sows aged about 10 months were collected from the slaughterhouse. When sampling, the mammary glands with better teat development were selected. (100X)) in DPBS preservation solution, and bring it back to the laboratory for processing within 8 hours;

[0025] Cut the breast with surgical scissors to find the breast tissue part. When looking for the breast tissue part, after the breast is cut open, there will be cortex and fat layer from the outside to the inside, and then there will be many lobular alveolar tissues inside, and there will be Tissues with a substantial structure, select the tissue with a substantial structure for the isolation and culture of breast cells, cut off a sufficient amount of breast tissue, wash 2-3 times with DPBS containing 1XPSN, then transfer to a 10cm culture dish, and use another Sterilized surgical scissors cut into small pieces of 1mm3, and can also be cut into pieces in sterile 5ml small tubes;

[0026...

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Abstract

The invention relates to a method for primary isolated culture of porcine mammary epithelial cells (PMEC). The method includes the following steps: (1) performing sampling; (2) performing pretreatment; (3) performing isolation; (4) performing culture; (5) performing subculturing; and (6) performing cell purification. The beneficial effects of the method are as follows: complex environment in vivocan be avoided by establishing a porcine mammary epithelial cell model in vitro; researches on the influences of specific conditions and treatment on the growing status of cells can be carried out without being affected by individual difference; and mammary epithelial cell cultures can be taken as models to research bio-mechanism and disease mechanism in mammary gland organs.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for primary isolation and culture of sow mammary gland epithelial cells (PMEC). Background technique [0002] The mammary gland is an accessory gland of the skin and is a specialized organ of the mother mammal. It can provide young animals with milk that contains various nutrients needed for growth and immunity. The good development of sow mammary gland tissue and the normal initiation of lactation function are of great significance to the growth and development of piglets and the exertion of their growth potential. Mammary gland epithelial cells have secretory function and play an important role in milk synthesis. Understanding the properties of mammary epithelial cells can help us understand the pathology of breast-related diseases such as breast cancer and identify new targets for treatment. Taking mammary gland epithelial cells as a model, fully understanding the molecular ...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0631C12N2509/00
Inventor 陈浩东刘望宏
Owner 陈浩东
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