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Canine parvovirus egg yolk antibody and preparation method thereof

A technology of canine parvovirus and egg yolk antibody, which is applied in the field of canine parvovirus egg yolk antibody and its preparation, can solve the problems of insufficient egg yolk antibody products and inability to prevent and treat diseases, and achieve good application prospects, low production cost, and high specificity Effect

Active Publication Date: 2022-06-03
HENGYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current CPV-related yolk antibody products are still not abundant, and cannot cope with the prevention and treatment of diseases caused by different epidemic strains in different regions

Method used

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  • Canine parvovirus egg yolk antibody and preparation method thereof
  • Canine parvovirus egg yolk antibody and preparation method thereof
  • Canine parvovirus egg yolk antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In step d, after concentrating the virus liquid prepared in step c, add 0.2% formaldehyde to inactivate at 37°C for 24 hours to obtain

[0043] wherein the adjuvant is Freund's complete adjuvant or Freund's incomplete adjuvant.

Embodiment 2

[0049] Adopt agarose diffusion test and indirect ELISA to detect the titer of egg yolk antibody.

Embodiment 3

[0053]

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Abstract

The present invention prepares egg yolk antibody based on a strong strain CPV‑HuN1703 strain with strong pathogenicity and good immune performance. Egg yolk antibodies prepared from other isolated strains. The yolk antibody has the advantages of safety, high specificity, good therapeutic effect, low production cost, etc., can effectively prevent and treat diseases caused by canine parvovirus, and has good application prospects.

Description

A kind of canine parvovirus egg yolk antibody and preparation method thereof Technical field: The invention belongs to the field of veterinary biological products, be specifically related to a kind of canine parvovirus egg yolk antibody and preparation method thereof Law. Background technology: Canine parvovirus disease is caused by canine parvovirus (Canine parvovirus, CPV) infection with vomiting, Highly contagious, acute, severe infectious disease characterized by diarrhea, leukopenia, and hemorrhagic enteritis, canine parvovirus (CPV) disease has been one of the most serious infectious diseases that endanger canine health since its discovery, especially for young children aged 2 to 6 months. Dogs are the most dangerous. Canine parvovirus is distributed around the world and can infect canines and mustelids, with an incidence of up to 70%‑100 %, the mortality rate is as high as 50%. With the improvement of living standards in my country, the number of police dog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/08C07K16/02C12N7/00A61K39/42A61P31/20
CPCC07K16/081C07K16/02C12N7/00A61P31/20C07K2317/76C12N2750/14351C12N2750/14363A61K2039/552A61K2039/505
Inventor 唐青海杨海杨灿王芳宇何丽芳刘会敬唐姣玉
Owner HENGYANG NORMAL UNIV
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