A rapid breeding method for high-yield and disease-resistant mango saplings

Abstract

The invention provides a high-yielding, rapid breeding method for disease-resistant mango saplings, comprising: (1) selecting a mango mutant individual plant; Pre-cultivate in the dark in silver 1 / 2MS medium, transfer to containing polyvinylpyrrolidone, TDZ and Na 2 S 2 o 3 (3) transfer to the seedling hardening bed for hardening culture, and when the seedlings grow to 10-15cm high, transfer to the nutrient cup containing the strengthening matrix of hardening seedlings Medium-intensified seedling hardening treatment; (4) placed at room temperature and cultivated to plant height ≥ 35cm, planted in the field, and selected a single plant with stable genetic and excellent traits as a new variety; the rapid breeding method of the present invention not only effectively eliminates mango species The impact of phenol damage in the seedling tissue culture process, and significantly improve the transplanting survival rate and growth quality of mango seedlings, speed up the selection and popularization of mango seedlings.

Description

technical field [0001] The invention relates to the technical field of mango breeding, in particular to a rapid breeding method for high-yield and disease-resistant mango saplings. Background technique [0002] Mango is one of the famous fruits in the tropics and subtropics. Mango cultivation areas are mainly distributed in Hainan, Guangxi, Guangdong, Yunnan and the dry-hot valley of the Jinsha River in Sichuan, which has high economic and social value. In the field of selective breeding of mangoes, different degrees of research have been carried out from seed selection breeding, artificial cross breeding to mutation breeding, and some effective results have been achieved. However, because mangoes are highly heterozygous woody plants, the The survival period is short, the traditional breeding method is difficult to improve mango varieties, and the cycle is long; however, the research on the method of mango seedling selection by tissue culture is quite weak, which often has s...

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Problems solved by technology

In the field of selective breeding of mangoes, different degrees of research have been carried out from seed selection breeding, artificial cross breeding to mutation breeding, and some effective results have been achieved. However, because mangoes are highly heterozygous woody plants, the The survival period is short, the traditional breeding method is difficult to improve mango varieties, and the cycle is long; however, the research on the method of mango seedling selection by tissue culture is quite weak, which often has serious phenol damage during the tissue culture process and the survival of the tissue culture seedlings after transplanting. Therefore, to find a rapid breeding method for high-yield, disease-resistant mango seedlings, to establish a reliable and efficient high-yield, rapid breeding process for disease-resistant mango seedling plants, for the rapid selection and breeding of excellent mango varieties It is important to promote the use of

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