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A kind of mixing device for dna extraction

A mixing and rotating shaft technology, which is applied in the direction of DNA preparation, recombinant DNA technology, mixer with rotating stirring device, etc., can solve the problems of increasing the work intensity of testing personnel and the difficulty of ensuring the mixing effect, so as to facilitate DNA extraction, Good mixing effect and reduced working intensity

Active Publication Date: 2022-07-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is generally dependent on the inspectors for manual mixing, which greatly increases the work intensity of the inspectors, and it is difficult to guarantee the mixing effect.

Method used

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  • A kind of mixing device for dna extraction
  • A kind of mixing device for dna extraction
  • A kind of mixing device for dna extraction

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Embodiment Construction

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] see Figures 1 to 7, In the embodiment of the present invention, a mixing device for DNA extraction includes a mixing box 1, a first valve 2, a first feeding funnel 3, a sealing cover 4, a temperature regulating box 5, a first motor 6, a first Pulley 7, second pulley 8, second valve 9, second feed funnel 10, stirring shaft 11, first splint 12, switch 13, first mixing device 14, third valve 15, fourth valve 16, first A vertical...

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Abstract

The invention discloses a mixing device for DNA extraction, which comprises a first motor, a water pump, a mixing blade, a temperature regulating box and a mixing box. The rotation of the first motor drives the rotation of the first pulley, thereby driving the rotation of the second pulley. , drive the stirring blade on the stirring shaft to rotate, so as to realize the mixing of the solution in the mixing box. The mixing blade rotates together during the rotation of the stirring blade, thereby improving the mixing effect of the solution in the mixing box. The heat-conducting medium is used to pass through the heat-conducting pipe, so as to realize the temperature adjustment of the solution in the temperature-adjusting box. The solution in the temperature-adjusting box further enters the mixing box through the liquid-conducting pipe, without the need for manual mixing by the inspectors, which greatly reduces the work intensity of the inspectors. After the mixing is completed, place the container such as the test tube between the first splint and the second splint, and the second splint moves towards the first splint under the action of the spring force, thereby realizing the clamping of the container such as the test tube. Limit, convenient for testing personnel to perform DNA extraction.

Description

technical field [0001] The invention relates to a mixing device, in particular to a mixing device for DNA extraction. Background technique [0002] DNA extraction is mainly CTAB method, and other methods include physical methods such as glass bead method, ultrasonic method, grinding method, freeze-thaw method. Chemical methods such as guanidine isothiocyanate method and alkali cracking method. Biological method: Enzymatic method. According to the different methods of nucleic acid separation and purification, there are siliceous materials, anion exchange resins, etc. The extraction methods of genomic DNA of different organisms (plants, animals, microorganisms) are different, but mainly solutions such as amyl alcohol, amyl alcohol and chloroform are added and further mixed. [0003] At present, manual mixing is generally performed by inspectors, which greatly increases the work intensity of inspectors, and the mixing effect is difficult to guarantee. SUMMARY OF THE INVENT...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10B01F33/50B01F35/32B01F35/92B01F27/906B01F27/95
CPCC12N15/1003B01F27/85B01F27/906B01F33/501B01F35/2215B01F35/4111B01F2035/99B01F35/92
Inventor 林风武赵岩孙宏斌张文龙王振兴程燊薛锦儒许哲男刘政甲李琳邹青旭郭博王晨
Owner JILIN UNIV
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