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A kind of hepatocellular carcinoma marker and detection kit

A technology of hepatocellular carcinoma and reagents, which is applied in the field of biomedicine, can solve the problems that the expression level of transcription or translation products is easily interfered by experimental operation, detection system error, and large amount of detection tissue, so as to avoid experimental operation error and system error, The effect of short detection time and small tissue demand

Active Publication Date: 2022-08-09
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, most of the current detection of molecular markers has shortcomings such as the need for a large amount of detection tissue, the slow detection cycle, the expression level of transcription or translation products is easily interfered by experimental operations, and the detection system has large errors, so it cannot be fully applied in clinical practice.

Method used

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  • A kind of hepatocellular carcinoma marker and detection kit
  • A kind of hepatocellular carcinoma marker and detection kit
  • A kind of hepatocellular carcinoma marker and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0051] We conducted miRNA and mRNA analysis on normal liver (NH) tissue and liver cancer (HCC) tissue from 7 patients with liver cancer (from: Tangdu Hospital of Air Force Military Medical University, signed informed consent and related contracts to ensure legal compliance with the use process) Sequencing to identify the miR-144 / miR-451a cluster as a candidate.

[0052] The specific implementation steps are as follows:

[0053] 1) Use surgery or biopsy to obtain liver tissue specimens of the individual to be tested, about 2mm x 2mm x 1mm in size;

[0054] 2) Use proteinase K-containing tissue lysis solution (about 500 μl) to lyse the collected tissue, and digest at 55°C for 6 hours;

[0055] 3) After carefully blowing and mixing the fully lysed tissue lysate, centrifuge at a low temperature and at a height of 12,000g for 10 minutes at 4°C to fully precipitate tissue and cell debris;

[0056] 4) Carefully aspirate the supernatant, add an equal volume of Tris-saturated phenol ...

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Abstract

The present invention provides a new marker for hepatocellular carcinoma and a detection kit. Based on the marker, early diagnosis of hepatocellular carcinoma can be performed more efficiently and accurately. The tissue demand is small, the detection time is short, the detection accuracy is high, and the operation The method is simple and the cost is low. More importantly, the present invention comprehensively considers the interaction between liver cancer and liver cancer immune microenvironment, and provides further treatment strategies for liver cancer patients, such as targeting innate immune cells or targeting T lymphocytes while providing early diagnostic markers for liver cancer. Treatment selection provides good reference and evaluation indicators. The invention has high reference value for early diagnosis of liver cancer and selection of immunotherapy, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a marker for hepatocellular carcinoma and a detection kit. Background technique [0002] Hepatocellular carcinoma (HCC) is the most common malignant tumor of digestive system, with poor prognosis and increasing mortality. A variety of risk factors may lead to genetic or phenotypic changes and contribute to the progression of liver cancer, including viruses, fibrosis, metabolic disorders, and alcohol. The complexity of etiology increases the difficulty of fully understanding the molecular mechanisms of HCC. [0003] According to the expression of specific molecular markers, auxiliary diagnosis of hepatocellular carcinoma not only greatly improves the diagnostic accuracy of hepatocellular carcinoma and shortens the diagnosis time of hepatocellular carcinoma, but also allows molecular grading of hepatocellular carcinoma through molecular diagnosis. It is conducive to the rationa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/154C12Q2600/178
Inventor 刘雷赵俊龙李惠晨
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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