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Method for capturing genes related to cognitive disorders and movement disorders, and kit

A technology for movement disorders and cognitive disorders, which is applied in the fields of biochemical equipment and methods, biological tests, and microbial determination/inspection to achieve the effect of accurate sequencing of target genes and reduced sequencing costs.

Pending Publication Date: 2021-01-15
FUJIAN MEDICAL UNIV UNION HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no diagnostic test exists for the definitive diagnosis of the early stages of cognitive and motor impairment

Method used

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  • Method for capturing genes related to cognitive disorders and movement disorders, and kit
  • Method for capturing genes related to cognitive disorders and movement disorders, and kit
  • Method for capturing genes related to cognitive disorders and movement disorders, and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Capture Probe Set of Cognitive Impairment and Movement Disorder-Related Genes

[0045] The present embodiment provides a set of capture probes for capturing cognitive impairment and movement disorder-related genes, the genes captured by the capture probe set and the chromosomal coordinate information of the genes are shown in the following table:

[0046]

[0047]

[0048] The design of the capture probe group follows the following rules:

[0049] (a) The length of the probe is 120bp;

[0050] (b) probe design to merge adjacent regions;

[0051] (c) The probe design interval extends 20bp from the target interval to the two wings;

[0052] (d) The probe reference is the sense strand of the genome;

[0053] (e) the consensus sequence of both wings of the probe is shown in SEQ ID NO.1 and SEQ ID NO.2;

[0054] Numbering sequence(5'-3') SEQIDNO.1 GAAGCGAGGATCAACT SEQIDNO.2 CATTGCGTGAACCGA

[0055] (f) Ensure probe specificit...

Embodiment 2

[0058] Embodiment 2 is used for the kit of library construction

[0059] The present embodiment provides a kind of test kit of constructing the library of dementia-related genes, and this test kit comprises following reagent:

[0060] 1. the capture probe group of the dementia-associated gene that embodiment 1 provides, and this probe group is through biotin labeling; And be used for capturing hybridization target sequence through streptavidin-labeled magnetic beads

[0061] ② Adapters used for multi-sample hybridization capture are used to block HybBlock before library hybridization, and the kit is Agilent SureSelect QXT Library Prep Kit Genomic DNA Library Construction Kit.

[0062] 3. the kit also includes PCR amplification primers for enriching the captured target sequence: the PCR amplification primers have sequences as shown in SEQ ID NO.3 and SEQ ID NO.4:

[0063] Numbering sequence(5'-3') SEQ ID NO.3 GAAGCGAGGATCAACT SEQIDNO.4 TCGGTTCACGCAATG ...

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Abstract

The invention provides a capture probe group of genes related to cognitive disorders and movement disorders, a use method of a kit and application, and relates to the technical field of gene detection. The capture probe group comprises 69 gene coding regions, can perform capturing for specific coding regions and perform high-depth sequencing, so that a target gene can be sequenced accurately, andthe sequencing cost is reduced.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a method and a kit for capturing genes related to cognitive impairment and movement disorder, in particular to the detection of molecular markers in blood and its application in molecular diagnosis. Background technique [0002] Cognition is an intelligent processing process for the body to recognize and acquire knowledge, involving a series of random, psychological and social behaviors such as learning, memory, language, thinking, spirit, and emotion. Cognitive impairment refers to a pathological process in which abnormalities in the brain's high-level intellectual processing related to the above-mentioned learning, memory, and thinking judgments lead to severe learning and memory impairments, accompanied by changes such as aphasia, apraxia, agnosia, or aphasia. The most common disease that causes cognitive impairment is dementia caused by various diseases. Dementia has a high p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6806C40B50/06C12N15/11G01N33/68G01N33/543
CPCC12Q1/6883C12Q1/6806C40B50/06G01N33/6893G01N33/54306G01N2800/2835G01N2800/2814C12Q2600/158C12Q2600/156
Inventor 潘晓东陈晓春辛佳蔚余尔涵林俊含杨思宇朱志保
Owner FUJIAN MEDICAL UNIV UNION HOSPITAL
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