A gene encoding ga20ox-oxidase and its application

An oxidase and gene technology, applied in the field of genetic engineering, can solve the problems of reducing plant height and endogenous GA content, and achieve the effect of simple and efficient method

CN112251451BActive Publication Date: 2021-12-14GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2021-12-14

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Abstract

The invention belongs to the technical field of genetic engineering, in particular to a gene encoding GA20ox-oxidase and its application. A gene encoding GA20ox-oxidase is obtained from a constructed yeast cDNA library, and the nucleotide sequence of the gene is shown in SEQ ID No.1. In the invention, the GA20ox gene sequence is directly obtained from the test material, the physical and chemical activity of the gene is analyzed, and the gene is used as an index for regulating dwarfing traits. The present invention relates to the cloning of the GA20ox oxidase gene and the corresponding amino acid sequence analysis, and the determination of the expression of the GA20ox gene by a semi-fluorescent quantitative method provides a new method for early prediction of whether the test material has dwarfing traits; The method of measuring the expression level of GA20ox gene by the semi-fluorescent quantitative analysis method of the present invention is simple and efficient for the early prediction of whether the test material has dwarfing characteristics.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, in particular to a gene encoding GA20ox-oxidase and its application. Background technique

[0002] Dwarf planting is a constant development trend of crop production. Plant dwarfing is mainly divided into genetic dwarfing and physiological dwarfing. A single gene mutation belongs to hereditary dwarfing, and its dwarfing incentive is mostly gene synthesis protein, which affects hormones, and hormones affect phenotype; the influence of hand and environmental factors leads to changes in plant phenotype, which belongs to physiological dwarfing, and its dwarfing effect The mechanism is mostly that environmental changes affect the secretion of plant hormones, which eventually lead to changes. But no matter what kind of dwarfing mode, the phenotype must be affected by hormones, and the hormones in the process of plant growth are mostly related to gibberellin.

[0003] The study of dwarf ge...

Examples

Embodiment

[0024] 1. Carrier and competent state

[0025] The cloning vector pGE-T Vector was purchased from Huada Gene Biotechnology (Shenzhen) Co., Ltd.; JM109 competence was preserved in the Key Laboratory of New Technologies for Breeding and Comprehensive Development of Superior Crops, Guangxi Tropical Crops Research Institute; subcellular localization vectors pK7FWGF2.0 and PEASY -T1 was provided by Jiakang Chuangyin Biotechnology (Wuhan) Co., Ltd.

[0026] 2. Main Reagents

[0027] DNA Marker, T 4 DNA ligase, Q5 ® Ultra-fidelity DNA polymerase, 2×Taq Master Mix, FirstChoice RLM-RACE Kit, 5×RT Reaction Buffer, dNTP, RNaes Inhibitor, M-MLV RTase, gel recovery kit were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. The adapters and primers were synthesized by BGI Biotechnology (Shenzhen) Co., Ltd.

[0028] 3. Method