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Bovine embryo in-vitro early implantation culture system

A technology of bovine embryos and embryoid bodies, which is applied in the field of livestock reproduction, can solve the problems of lack of implantation models, and achieve the effect of early implantation of bovine embryos in vitro

Pending Publication Date: 2021-05-07
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is urgent to establish an early implantation culture system for bovine embryos in vitro, so as to solve the problem of lack of bovine embryo implantation models in vitro

Method used

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  • Bovine embryo in-vitro early implantation culture system
  • Bovine embryo in-vitro early implantation culture system

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Embodiment Construction

[0032] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, which are only used to explain the present invention rather than limit the protection scope of the present invention.

[0033] The invention provides a bovine embryo in vitro early implantation culture system, which is closer to the bovine embryo implantation environment in vivo, which makes it possible to observe the bovine embryo implantation in vitro and helps to study the mechanism of pregnancy failure. At the same time, the culture system can be used to carry out a large number of researches on the factors affecting embryo implantation, such as the research on drugs, hormones and poisons. It is an unparalleled tool that is of great significance to improving the economic benefits of the cattle industry.

[0034] 1. Experimental method

[0035] 1.1 Reagents and consumables

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Abstract

The invention discloses a bovine embryo in-vitro early implantation culture system. A Transwell chamber with an ultra-microporous film at the bottom is used as an upper chamber for culturing embryos, culture holes of a 6-hole cell culture plate are used as a lower chamber for culturing endometrial cells, and the ultra-microporous film of the Transwell chamber allows the embryos to secrete and absorb various nutrient substances in an all-dimensional and three-dimensional manner, so that metabolism and activity are carried out in a manner close to nature. According to the staining result of the binuclear, trinuclear or multinuclear cells in a lower indoor endometrial cell layer, the early implantation capacity of the embryos can be judged, so that the pregnancy efficiency is improved.

Description

technical field [0001] The invention relates to the field of livestock reproduction, in particular to a bovine embryo in vitro early implantation culture system. Background technique [0002] Embryo implantation in cattle starts from the 8th to 10th day of fertilization, when the embryo hatches from the zona pellucida to form a hatching embryo. The hatching embryo consists of an inner mass of cells that can form individuals and an outer layer of trophoblast cells. The trophoblast forms the fetal membrane of the blastoderm and plays a central role in the implantation process: after the early bovine embryo hatches from the zona pellucida, it is localized to the endometrial epithelium by trophoblast cells, and at the same time, trophoblast cells divide the cytoplasm by nuclear division Do multiple mitosis in a non-dividing manner to form giant binuclear cells, which migrate to the microvilli protrusions of the endometrium and fuse with mononuclear endometrial cells to produce ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M3/06C12N5/073C12N5/071
CPCC12M21/06C12M25/04C12M25/14C12M29/04C12M35/08C12N5/0604C12N2502/243C12N2501/392C12N2501/24
Inventor 华松付明哲王平孙明坤
Owner NORTHWEST A & F UNIV
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