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Application of vascular carrier in immobilization culture of filamentous microorganisms

A technology of immobilized culture and filamentous microorganisms, applied in the direction of being immobilized on/in an organic carrier, can solve the problems of biofilm shedding, affecting the growth and metabolism of immobilized microorganisms, etc., to maintain growth, shorten the growth cycle, Not easy to fall off effect

Inactive Publication Date: 2014-08-27
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive aeration produces shear force, which often peels off the biofilm and affects the growth and metabolism of immobilized microorganisms.

Method used

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  • Application of vascular carrier in immobilization culture of filamentous microorganisms
  • Application of vascular carrier in immobilization culture of filamentous microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0037] Example 1: Preparation of vascular carrier and its promotion of growth of white rot fungi

[0038] (1) Fabrication of vascular carrier

[0039] The vascular carrier is made of a Velcro material to make a hollow tubular carrier (fold it in half and suture the overlapping sides or suture the corresponding two sides of the two Velcro strips together to form a tubular structure), the carrier is open at both ends or one end is open, and the opening The ends are supported with a tubular material (plastic tube) and closed with a permeable material (cotton) that is permeable to nutrients and oxygen impermeable to microorganisms. Both the Velcro fabric with small hooks and the Velcro fabric with pile loops can be used to make vascular carriers, and the carrier made of Velcro fabrics with small hooks is shown in figure 1 a and figure 1 As shown in c, the carrier of the Velcro fabric with loops is shown in figure 1 b and figure 1 as shown in d.

[0040] (2) Vascular carri...

Embodiment 2

[0049] Example 2: Vascular carriers promote the synthesis of manganese peroxidase in white rot fungi

[0050] The vascular carrier is made of a Velcro material to make a hollow tubular carrier (fold it in half and suture the overlapping sides or suture the corresponding two sides of the two Velcro strips together to form a tubular structure, with Velcro on the surface of the loop), Both ends of the carrier are open, and the open end is supported by a tubular material (plexiglass tube) and sealed with a permeable material (cotton cloth) that can permeate nutrients and oxygen but impervious to microorganisms (see figure 1 b);

[0051] Into 100 ml Bonnarme broth (Bonnarme P, Jeffries TW (1990) Mn(II) regulation of lignin peroxidases and Manganese-dependent peroxidases from lignin-degrading white rot fungi. Appl. Environ. Microbiol. 56: 210–217) Five vascular carriers made of Velcro with looped surface and no vascular carrier were treated, sterilized at 121°C for 30 min; then in...

Embodiment 3

[0053] Embodiment 3: Vascular carrier promotes Trichoderma harzianum ( Trichoderma harzianum ) growth

[0054] The vascular carrier is made of Velcro material to make a hollow tubular carrier (fold it in half and sew its overlapping sides together or sew the corresponding two sides of two Velcro strips together to form a tubular structure, Velcro with looped fabric surface) , one end of the carrier is open, and the open end is supported by a tubular material (stainless steel tube), and sealed with a permeable material (rubber plug) that can pass nutrients and oxygen but not microorganisms;

[0055] In 100 ml medium (glucose: 10 g / L, NaNO 3 : 10 g / L, MnSO 4 : 0.5 g / L, KH 2 PO 4 : 2g / L), respectively add 0, 3, 4, 5 vascular carriers made of Velcro with loop surface, and sterilize at 121°C for 30 minutes; then inoculate Trichoderma harzianum ( T. harzianum ) spores, inoculate 1×10 per ml 6 The spores were then placed at 37°C and cultured on a shaker at 100 rpm for 5 day...

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Abstract

The invention discloses an application of a vascular carrier in immobilization culture of filamentous microorganisms. The vascular carrier is a hollow tubular carrier made of a fastening tape material; one end or both ends of the carrier can be open; the opening end is supported by a tabular material with certain supporting effect; the opening of the tabular material with certain supporting effect is sealed by a permeable material to allow culture solutions to pass through while spores of bacteria or fungi not to pass through; the most characteristic of the carrier is that nutrients and oxygen can pass through the carrier opening to penetrate into the inside of the carrier, and to penetrate and diffuse from the inside to the outside through the inner wall of the carrier, so as to provide nutrients and oxygen for mycelia attached outside the carrier and to promote the growth of the mycelia; the carrier solves the problem, for common carriers, of biofilm stripping and shedding due to nutrient and oxygen deficiency of the inside of mycelia biofilms, can also promote the attachment and growth of filamentous microorganisms, shortens the growth period, improves the growth, promotes the metabolism, and increases the enzyme production amount of filamentous microorganisms.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms, and in particular relates to a carrier for immobilizing and cultivating filamentous microorganisms—a Velcro vascular carrier, which can promote the growth and metabolism of filamentous microorganisms. Background technique [0002] In the cultivation of microorganisms, people often use immobilized culture, because immobilized culture can promote the growth and metabolism of microorganisms. [0003] The immobilized culture of white rot fungi is conducive to the maintenance of their activity. Saucedo et al. (1988) in the Gibberella fujikuroi The study on the immobilization of mycelium found that immobilized culture can improve the stability of fungal growth and prolong the time of maintaining its activity; Bottcher et al. (1988) and Komel et al. (1985) obtained similar results in the study of immobilized fungi. [0004] Immobilized white-rot fungi can promote their synthesis of metabolic e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02
Inventor 周成董明于晓龙陈宇琳邓志华
Owner KUNMING UNIV OF SCI & TECH
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