Myeloid tissue decalcification solution and method for preparing myeloid tissue paraffin sections

Abstract

The invention provides a myeloid tissue decalcification solution and a method for preparing myeloid tissue paraffin sections. The myeloid tissue decalcification solution is composed of hydrochloric acid, formaldehyde and distilled water, wherein the volume percentage concentration of the hydrochloric acid in the myeloid tissue decalcification solution is 1-3%, and the volume percentage concentration of the formaldehyde in the myeloid tissue decalcification solution is 9-11%. According to the myeloid tissue decalcification solution and the method for preparing the myeloid tissue paraffin sections, the myeloid tissue decalcification solution is used for decalcifying a myeloid tissue specimen, the specimen pretreatment process is shortened, the report time is short, timely diagnosis is facilitated, and the obtained HE section shows that the cell morphology is well preserved and the tissue structure is clear; the immunohistochemical staining sections show that the staining effect is excellent, the positioning is accurate, and the background is clean; and and the phenomenon of excessive decalcification of the sample is avoided.

Description

technical field [0001] The invention relates to the technical field of pathological detection, in particular to a bone marrow tissue decalcification solution and a method for making paraffin sections of bone marrow tissue. Background technique [0002] Bone marrow biopsy is one of the important components of pathological examination, and its diagnosis report is an important basis for the diagnosis and treatment of diseases in clinical departments such as hematology. Currently, there are two main methods for embedding bone marrow biopsy specimens, namely the plastic method and the paraffin method. The plastic method has not been popularized so far due to various processing procedures, easy loss of antigenic activity, and difficulty in immunohistochemical staining. The paraffin method is mainly used. Mainly. [0003] The most important link in making high-quality bone marrow slices by paraffin method is the fixation and decalcification of the specimen tissue. If the bone mar...

AI Technical Summary

Problems solved by technology

However, EDTA chelation decalcification and organic weak acid decalcification take a long time, which affects the timeliness of treatment; electrolytic decalcification requires special equipment, which is expensive and inconvenient; inorganic strong acid decalcification generally has a faster decalcification speed, and the time is not easy to control. Continuous acupuncture observation is required, which is prone to excessive decalcification and may lead to the loss of many antigens, which has a significant impact on immunohistochemical staining; mixed inorganic strong acid and organic acid decalcification requires residual acid treatment

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