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Aspergillus flavus strains and uses thereof

A technology of Aspergillus purpureus and Aspergillus purpura, applied in the field of microbial preparations, can solve the problems of poor nutrition, unfavorable plant growth, unfavorable soil microbial reproduction, etc., and achieve the effects of promoting growth, improving population structure, and increasing crop biomass

Active Publication Date: 2022-06-28
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the adversity conditions of relatively immature soils are neither conducive to plant growth nor to the reproduction of soil microorganisms due to poor nutrition and too low pH. So far, there has been no research on the use of plant growth-promoting fungi to promote plant growth and development in immature soil conditions. Related news about soil improvement

Method used

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  • Aspergillus flavus strains and uses thereof
  • Aspergillus flavus strains and uses thereof
  • Aspergillus flavus strains and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, purification, screening and identification of the isolated line of embodiment 1

[0027] 1. Isolation and Purification

[0028] The soil of barren land under the forest in Sanmen County, Taizhou City, Zhejiang Province was collected by the 5-point sampling method, put in a clean sealed bag, and brought back to the laboratory. Soil microbial strains were isolated by soil dilution method. That is, weigh 2g of soil sample in 18mL of double distilled water (ddH 2 (0), shake on a shaker at 180rpm for 30min to make 10 -1 Soil dilution; take 1mL of this dilution to contain 9mL ddH 2 O centrifuge tubes, made of 10 -2 soil dilution; and so on, to get 10 -3 、10 -4 Soil dilution. Take 100μL of 10 -3 and 10 -4 Soil dilution, spread on the potato dextrose agar medium (PDA) plate, and place it in a 25°C incubator for 3 to 4 days, and select the colony with fast growth rate (large colony) and different colony shapes (for example, color, Sparse or dense hyphae, con...

Embodiment 2

[0058] Embodiment 2 Determination of phosphorus dissolving ability

[0059] The isolates HZ23, P. oxalicum HZ06, A. brunneoviolaceus HZ10, and A. tubingensis HZ123 preserved at -70°C were placed on potato dextrose agar medium ( PDA) plate was cultured and activated at 25°C for 3 days;

[0060] Bacteria slices (5 mm in diameter) were cut out with a hole punch and transferred to NBRI-MTSB medium plates, cultured at 25°C for 3 days, and the diameter of the phosphorus-dissolving circle was measured.

[0061] see Figure 4 , the experimental results show that the diameter of the phosphorus-dissolving circle of the separation system HZ23 is 3.88±0.25cm ( Figure 4 B), although it is larger than Aspergillus flavus HZ10 (3.60±0.18cm) ( Figure 4 C) and Penicillium oxalicum (Penicillium oxalicum) HZ06 (3.48±0.05cm) ( Figure 4 A), but significantly smaller than the diameter of the phosphorus-dissolving circle of Aspergillus tubingensis (A. tubingensis) HZ123 (4.20±0.21cm) ( Figur...

Embodiment 3

[0062] Example 3 Determination of Siderophore Ability

[0063] The four strains activated in Example 2 were respectively cut out with a puncher to cut bacterial slices (5 mm in diameter) and transferred to CAS medium plates and cultured at 25° C. for 3 days, and the diameter of the orange-yellow halo was measured.

[0064] see Figure 5 , the diameter of the orange-yellow halo of the separation system HZ23 is 1.23±0.39cm ( Figure 5 B), larger than Aspergillus fusiformis HZ10 (1.04±0.24cm) ( Figure 5 C), but significantly smaller than Aspergillus tubingensis HZ123 (2.78±0.71cm) ( Figure 5 D) and Penicillium oxalicum HZ06 (2.05±0.56cm) ( Figure 5 A).

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Abstract

The invention discloses a strain of Aspergillus frugosa and its application. The strain of Aspergillus brunneoviolaceus is named Aspergillus brunneoviolaceus HZ23, and the preservation number is CCTCC M 2021173. The described Aspergillus fusicolor strain has the ability to efficiently dissolve phosphorus, produce siderophores and 3-indoleacetic acid (IAA), and can be used for agricultural production of newly reclaimed cultivated land, improve the structure of soil microbial populations, and promote soil maturation, thereby promoting the growth of crops , increase crop biomass.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to Aspergillus flavus strains and uses thereof. Background technique [0002] In order to alleviate the problems of decreasing existing cultivated land resources and the increasing difficulty of replenishing and balancing the occupation of cultivated land, many places in my country use newly reclaimed cultivated land to fill the area of ​​occupied cultivated land to ensure the relative stability of the total amount of local cultivated land. However, freshly cultivated land differs from arable land in that it typically has immature soils, which in many cases are high in gravel, acidic and nutrient-poor, making them unsuitable for crop growth. People often use livestock and poultry manure, biosolids, pulp by-products, plant residues and crop residues for soil improvement to change soil physical and chemical properties and microbial community structure. Among them, soil microorga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/34A01P21/00C05G3/00C05G3/80C12R1/66
CPCC12N1/14A01N63/34C05G3/00C05G3/80
Inventor 李戌清严建立张敬泽李丁圯
Owner HANGZHOU ACAD OF AGRI SCI
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