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Hydroxidation bacterium flora with specific function as well as screening method and application thereof

A technology of hydrogen oxidizing bacteria and flora, applied in the field of microorganisms, can solve the problems of complicated process, inability to maximize production of hydrogen oxidizing bacteria, high production costs, etc., and achieve the effect of solving white pollution, avoiding environmental problems, and alleviating environmental problems

Active Publication Date: 2022-07-26
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods of the prior art require that the cultivation process cannot be disturbed and polluted by the external environment, the production cost is high, the process is complicated, and hydrogen oxidizing bacteria often cannot achieve its maximum production

Method used

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  • Hydroxidation bacterium flora with specific function as well as screening method and application thereof
  • Hydroxidation bacterium flora with specific function as well as screening method and application thereof
  • Hydroxidation bacterium flora with specific function as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Obtaining domesticated microflora

[0056] Activated sludge from a municipal sewage treatment plant in Chengdu was extracted and aged in the air for 48 hours. Take 5 g of the aged activated sludge and put it in a 250 mL anaerobic bottle, add 100 mL of enriched medium, place it in a shaker, and cultivate at 200 rpm and 30° C. for 3 days to obtain a culture solution. The culture medium was collected, and the inoculum amount of 10% (V / V) was transferred into a new enriched medium for subculture, and repeated 3 times to obtain enriched microbial flora.

[0057] The enriched microbial flora was inoculated into anaerobic flasks containing fermentation medium at 10% (V / V) inoculum for acclimation. Specifically, 90 mL of fermentation medium was added to the anaerobic bottle, 10 mL of enriched microbial flora was inoculated, and the remaining headspace was filled with a mixture of hydrogen, oxygen and carbon dioxide in a volume ratio of 8:1:1 and sealed. . After se...

Embodiment 2

[0059] Embodiment 2: a kind of method of screening the hydrogen oxidizing bacteria group that obtains high-yielding PHB

[0060] The reaction device used for screening the hydrogen-oxidizing bacteria group with specific functions involved in the present invention is a fermenter, and the specific structure is as follows: figure 1 shown. Described fermentation tank is glass fermentation tank, also comprises: 2 / CO 2 / O 2 gas cylinders, gas mixing devices to regulate gas flow, peristaltic pumps for pumping in and out of the culture medium, aeration devices, thermometers to control operating conditions, pH meters, agitators, and exhaust gas collection devices. In each embodiment including this embodiment, the effective volume of the fermentation tank is 3L. It should be understood that: the present invention figure 1 And the structure of the fermenter provided here is exemplary, and in practice, those skilled in the art can make adaptive adjustments according to actual needs....

Embodiment 3

[0067] Embodiment 3: a kind of method of screening to obtain the hydrogen oxidizing bacteria colony of high-yield single-cell protein

[0068] The conditions such as the reaction device and the screening medium used in this example are the same as those in the second example, the differences are: the total gas ratio, H 2 :O 2 =4:1. Controlled by flow, H 2 , O 2 , CO 2 The flow rates were 45.44mL / min, 11.36mL / min, and 12.63mL / min, respectively.

[0069] The biomass changes in this example are as follows Image 6 As shown, the change of ammonia nitrogen utilization is as follows Figure 7 As shown, the single-cell protein content is as follows Figure 8 As shown, the bacterial community structure is as follows Figure 9 shown.

[0070] After 10 days, sequencing and sequence alignment showed that the microbial flora structure obtained under the culture conditions of this example was: about 34% Achromobacter, about 25% Enterobacter, about 10% Dyella, about 6% Pandoraea, a...

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Abstract

The invention belongs to the field of microorganisms, and particularly relates to a hydrogen oxidizing bacterium flora with a specific function and a screening method and application thereof. According to the specific technical scheme, the hydrogen-oxidizing bacterium flora screening method capable of adjusting the types and the yield of the microbial products is realized by adjusting the use amount relation of hydrogen and oxygen in a culture environment. According to the novel method for screening the mixed flora of the hydrogen-oxidizing bacteria, by changing the gas proportion and the hydraulic retention time, the population structure of the hydrogen-oxidizing bacteria can be optimized, the mixed flora of high-yield single-cell protein and high-yield PHB can be obtained respectively, the population structure of the hydrogen-oxidizing bacteria is stably maintained, the requirement for the culture environment is not high, and even if a small amount of infectious microbes exist, the mixed flora can be still still still still. The method does not influence the implementation of the whole purpose of the invention, can effectively overcome the defects of hydrogen-oxidizing pure bacteria fermentation, realizes the regulation of the functions of the hydrogen-oxidizing bacteria flora and the efficient production of the product, and achieves the level equivalent to that of pure bacteria fermentation.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a hydrogen oxidizing bacteria group with specific functions and a screening method and application thereof. Background technique [0002] Hydrogen oxidizing bacteria (HOB) are a kind of 2 as an electron donor, with O 2 As an electron acceptor, assimilates CO 2 and ammonia nitrogen bacteria. Studies have shown that many high value-added products can be synthesized by using hydrogen oxidizing bacteria, such as PHB, single-cell protein, alcohols, terpenes, plant growth promoters, etc. [0003] At present, most of the reports on the utilization of hydrogen oxidizing bacteria are single hydrogen oxidizing bacteria. According to their own characteristics of different kinds of single hydrogen oxidizing bacteria, different high value-added products are produced by fermentation. In addition, there are also a few reports that several kinds of single hydrogen-oxidizing bacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/04C12N1/20C12R1/01C12R1/025
CPCC12N1/36C12N1/04C12N1/20
Inventor 李大平田畅张礼霞王浩然何晓红
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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