Detection kit for Sildenafil signal channel gene, detection method for detection kit and application of detection kit

Abstract

The invention discloses a detection kit for a Sildenafil signal channel gene, a detection method for the detection kit and application of the detection kit. According to the detection kit, specific amplification primers are designed in view of polymorphism of three genes GNB3, NOS3 and NOS1 and are applied to fluorescent quantitative PCR detection; and the kit comprises the following ingredients: a GNB3 reaction solution, a NOS3 reaction solution, a NOS1 reaction solution, a positive control, a blank control and an enzyme. According to the detection kit, the detection method and the application of the detection kit, the Sildenafil signal channel gene is detected by employing a fluorescent quantitative PCR method, and the kit can be used for simultaneously detecting 3 core loci; and through detecting genotypes of the core loci, i.e., GNB3 (C825T), NOS3 (T-786C) and NOS1 (C276T), whether a NO signal channel strengthening capability level of a sample source is normal or relatively high can be determined relatively directly, and thus, gene-aspect suggestions are provided for clinical customized medication.

Description

technical field [0001] The invention relates to a sildenafil signaling pathway gene detection kit, a detection method and an application thereof, and belongs to the field of gene detection. Background technique [0002] Erectile dysfunction (ED), clinically defined as the inability to achieve and maintain an erection sufficient to permit satisfactory sexual intercourse, has organic, psychological, and mixed etiologies. Although ED is a non-threatening disorder, it can affect physical and psychosocial health, with a major impact on the quality of life of patients and their partners. Risk of ED has been shown to be associated with comorbid conditions such as diabetes, obesity, cardiovascular disease, hypertension, and / or dyslipidemia, and may also be due to neurological damage following radical prostatectomy. [0003] Nitric oxide (NO), a key mediator involved in penile erection, is produced from l-arginine by different NO synthases (NOS), including neuronal (nNOS), endotheli...

AI Technical Summary

Problems solved by technology

At present, the polymorphisms of the three genes GNB3 (C825T), NOS3 (T-786C), and NOS1 (C276T) have not been detected to judge the clinical ED patients and ED patients after prostatectomy. Metabolism ability, so as to give a certain guidance for personalized medicine

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