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A biologically active protein for preventing and treating human papillomavirus infection, its preparation method and its application

A bioactive protein and human papillomavirus technology, applied in the field of medicine, can solve the problems of affecting the effect of anti-HPV virus, poor modification effect of lactoferrin, and large steric hindrance, so as to prevent the expansion of infection, low cost, The effect of small steric hindrance

Active Publication Date: 2022-07-08
江苏正大天创生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The LK protein used in this patent is to modify lactoferrin by 3-hydroxy-phthalic anhydride. Due to the large steric hindrance of 3-hydroxy-phthalic anhydride and low activity, the modification of lactoferrin The effect is not good, affecting the effect of anti-HPV virus

Method used

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  • A biologically active protein for preventing and treating human papillomavirus infection, its preparation method and its application
  • A biologically active protein for preventing and treating human papillomavirus infection, its preparation method and its application
  • A biologically active protein for preventing and treating human papillomavirus infection, its preparation method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of acid anhydride lactoferrin

[0037] In this embodiment, the preparation of acid anhydride lactoferrin includes the following steps: at a temperature of 25°C, dissolving 4-hydroxyphthalic anhydride in dimethyl sulfoxide to obtain a saturated 4-hydroxyphthalic anhydride solution; Ferritin was dissolved in 0.1M trisodium phosphate solution to obtain a protein solution with a final concentration of 30 mg / mL; the above 4-hydroxyphthalic anhydride solution was added to the protein solution, stirred and mixed, and the final concentration of 4-hydroxyphthalic anhydride in the reaction system is 60mM, and left to react for 2 hours; acid anhydride lactoferrin is obtained; after sterilization with 0.45uM filter membrane suction filtration, use pH 7.2 phosphate buffer to carry out ultrafiltration replacement purification, the ultrafiltration membrane molecular weight cut-off is 10KD; The 0.45uM microporous membrane was filtered and sterilized by suction, th...

Embodiment 2

[0039] Example 2 Preparation of acid anhydride lactoferrin

[0040] At a temperature of 35°C, 4-hydroxyphthalic anhydride was dissolved in dimethyl sulfoxide to obtain a saturated 4-hydroxyphthalic anhydride solution; lactoferrin was dissolved in a 0.1M trisodium phosphate solution to obtain a final concentration of 50mg / mL protein solution; add the above-mentioned 4-hydroxyphthalic anhydride solution into the protein solution, stir and mix, the final concentration of 4-hydroxyphthalic anhydride in the reaction system is 80mM, and stand for 2 hours to react; obtain acid anhydride lactoferrin; Use a 0.45uM filter membrane for suction filtration and sterilization, and then use a pH 7.2 phosphate buffer for ultrafiltration replacement and purification. The molecular weight cut-off of the ultrafiltration membrane used is 10KD; then use a 0.45uM microporous filter membrane for suction filtration and sterilization, and add the protective agent trehalose. Freeze-drying is performed b...

Embodiment 3

[0042] Example 3 Preparation of acid anhydride lactoferrin

[0043] At a temperature of 50 °C, 4-hydroxyphthalic anhydride was dissolved in dimethyl sulfoxide to obtain a saturated 4-hydroxyphthalic anhydride solution; lactoferrin was dissolved in a 0.1M trisodium phosphate solution to obtain a final concentration of 80mg / mL protein solution; add the above-mentioned 4-hydroxyphthalic anhydride solution into the protein solution, stir and mix, the final concentration of 4-hydroxyphthalic anhydride in the reaction system is 80mM, and stand for 2 hours to react; obtain acid anhydride lactoferrin; Use a 0.45uM filter membrane for suction filtration and sterilization, and then use a pH 7.2 phosphate buffer for ultrafiltration replacement and purification. The molecular weight cut-off of the ultrafiltration membrane used is 10KD; then use a 0.45uM microporous filter membrane for suction filtration and sterilization, and add the protective agent sucrose. The freeze-drying machine is ...

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Abstract

The present invention provides a biologically active protein for preventing and treating human papillomavirus infection, a preparation method and application thereof. Described biologically active protein is acid anhydride lactoferrin, acid anhydride whey protein or acid anhydride histone, and described acid anhydride adopts 4-hydroxyphthalic anhydride, the derivative of 4-hydroxyphthalic anhydride, succinic anhydride or maleic anhydride. Modified protein. The biologically active protein of the present invention has the advantages of blocking the HPV virus from invading cells, preventing the virus from expanding infection, antibacterial, antioxidative, safe, stable, convenient for storage and transportation, low cost, and the like.

Description

technical field [0001] The invention relates to a biologically active protein for preventing and treating human papilloma virus infection, a preparation method and application thereof, and belongs to the technical field of medicine. Background technique [0002] Human papilloma virus (HPV) is one of the few viruses clearly associated with human cancer, and its persistent infection is an important risk factor for cervical cancer. According to statistics, high-risk HPV DNA can be detected in more than 99% of all cervical cancer patients. Approximately 50% of sexually active women have had an HPV infection during their lifetime, and those with persistent high-risk HPV infection have a 100- to 300-fold increased risk of cervical cancer. Therefore, early intervention and clearance of HPV infection are very important for the prevention of cervical lesions CIN. At present, there are many treatments for HPV infection, of which topical medication is one of the commonly used methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K38/40A61K38/17A61K47/32A61P31/20A61P35/00
CPCA61K47/54A61K38/40A61K38/1709A61K47/32A61P31/20A61P35/00A61K9/06A61K9/08A61K47/10A61K9/0034A61K9/02A61P31/00A61K38/018A61K38/17C07K14/79
Inventor 王曙蒙
Owner 江苏正大天创生物工程有限公司
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