Biological agent and its application in the preparation of drugs for preventing and controlling Ebola virus

Abstract

The invention discloses a biological preparation for preventing and controlling various genotypes of Ebola virus infection, which is prepared by the following steps: preparing an acid anhydride with dimethyl sulfoxide into a mother liquor with a concentration of 1M; preparing 0.1M dihydrogen phosphate Sodium solution, used to prepare the protein solution, the protein concentration is 20mg / ml; add the acid anhydride solution to the protein solution to be modified, so that the final concentration of the added acid anhydride is 12mM, after fully mixing, adjust the pH of the protein solution to 9.0, 25°C Incubate for 20 minutes; repeat the above process of mixing the acid anhydride and the protein solution, and finally make the final concentration of the acid anhydride in the protein solution 60 mM, the solution pH 9.0, and incubate at 25°C for 2 hours to complete the acid anhydride reaction; put the acid anhydride protein solution into the dialysis bag , dialyzed in pH 7.4 phosphate buffer for 48 hours, filtered the anhydrided protein solution with a 0.45 μm microporous membrane, and stored at 4°C.

Description

technical field [0001] The invention relates to a novel antiviral biological agent in the field of medical technology, in particular to a biological agent for preventing and controlling Ebola virus infection. Background technique [0002] Ebola virus disease (EVD) is a viral infectious disease caused by Ebola virus (EBOV) infection and transmitted through body fluids or close contact. The natural host of Ebola virus is fruit bats, which are mainly pathogenic to primates including humans. Since the discovery of Ebola virus in the 1970s, the virus infection has been sporadic in Central Africa Local outbreak, but the Ebola outbreak in West Africa that spread from 2014 to this year far exceeds any outbreak in history. According to WHO statistics, as of April 8, 2015, the total number of infected people was 25,550, of which more than 10,587 died . The incubation period of the disease is 2 to 21 days. The early symptoms are fever, gastrointestinal discomfort, muscle pain, etc. A...

AI Technical Summary

Problems solved by technology

First of all, ZMapp is composed of three neutralizing antibodies against Zaire-type Ebola virus. Whether it can effectively protect against mutated antigens or other types of Ebola virus in possible outbreaks in the future has yet to be determined. Second, the production process of ZMapp is relatively complicated, the cost is high, and it requires relatively complete storage and transportation conditions. Therefore, how to use it for epidemic control in countries with relatively backward economies in Africa is also a difficult problem

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