Preparation method for biological agent for preventing and controlling human papilloma virus infection
A technology of human papillomavirus and biological preparations, which is applied in the field of preparation of biological preparations, can solve the problems of unclear curative effect, deterioration of the immune system, expansion, etc., and achieve the effect of preventing the expansion of infection, simple preparation method, safe and toxic side effects
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experiment example 1
[0022] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:
[0023] (1) Separation and purification of β-lactoglobulin-based stable proteins from milk products;
[0024] (2) Use active acid anhydride to acid anhydride the separated and purified protein, and then purify the active protein again after dialysis;
[0025] 3-Hydroxy-phthalic anhydride (HP) was first dissolved in dimethylsulfoxide DMSO to a saturated concentration, then β-lactoglobulin (β-LG) was dissolved in pH 8.5, 0.1M sodium phosphate In the solution, make the final protein concentration 20mg / mL, then add the above-mentioned HP into the protein solution, oscillate and mix well, place it for 1 hour at a temperature of 25°C, dialyze with pH7.4 PBS, and then use 0.45uM microporous Sterilize by filter membrane, store at 4°C, and then make a gel according to the existing method.
experiment example 2
[0027] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:
[0028] First, dissolve HP in dimethyl sulfoxide DMSO until the concentration reaches saturation, then dissolve chicken serum albumin OVA in pH 8.5, 0.1M sodium phosphate solution, so that the final protein concentration is 20 mg / mL, and then dissolve the above HP solution Divide into five equal parts, add to the protein solution every 12 minutes, vortex and mix well, adjust the pH to 8.5 with 1M NaOH, and the final concentration of acid anhydride in the reaction system is 60mM. At a temperature of 25°C, place it for 1 hour, dialyze the pH7.4 phosphate buffer solution PBS at 4°C overnight, then filter and sterilize with a 0.45uM microporous membrane, store at 4°C, and then make a paste according to the existing method cream.
experiment example 3
[0030] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:
[0031] Firstly, HP was dissolved in dimethyl sulfoxide DMSO, the concentration reached saturation (1.19M), then rabbit serum protein RSA was dissolved in pH8.5, 0.1M sodium phosphate solution, so that the final protein concentration was 20mg / mL, and then The above HP solution was divided into five equal parts, added to the protein solution every 12 minutes, oscillated and mixed, 1M NaOH was used to adjust the pH to 8.5, and the final concentration of acid anhydride in the reaction system was 60 mM. At a temperature of 25°C, place it for 1 hour, dialyze the pH7.4 phosphate buffer solution PBS at 4 degrees overnight, then filter and sterilize it with a 0.45uM microporous membrane, store it at 4°C, and then make a spray according to the existing method agent.
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