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Preparation method for biological agent for preventing and controlling human papilloma virus infection

A technology of human papillomavirus and biological preparations, which is applied in the field of preparation of biological preparations, can solve the problems of unclear curative effect, deterioration of the immune system, expansion, etc., and achieve the effect of preventing the expansion of infection, simple preparation method, safe and toxic side effects

Active Publication Date: 2013-08-07
SHANXI JINBO BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, when the virus matures, it will be released from the cells, thereby infecting new cells, causing more infections and lesions in the mouth, hands and feet, and male and female genitals, and finally leading to the expansion and deterioration of the disease, and finally the immune system cannot clear it and form a sharp Condyloma, cervical erosion, chronic cervicitis and even tumor
At present, there is still a relative lack of drugs that can effectively prevent and control HPV virus infection in the world. It is recognized that the most effective vaccines are mainly preventive vaccines. It has been clinically confirmed that HPV vaccines can almost 100% prevent some HPV virus strains from infecting, but it Does not prevent infection with all types of virus strains and is expensive
In terms of treatment, the immunomodulatory drugs used because there are no specific drugs are generally unable to effectively prevent HPV infection, have unclear curative effects, and are expensive, which greatly limits their clinical use, especially in low-income countries. developing countries
[0005] Therefore, great enthusiasm has been poured into the research and development of new drugs and biological agents that have preventive and control effects on a variety of HPV strains, but there has been no breakthrough.

Method used

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  • Preparation method for biological agent for preventing and controlling human papilloma virus infection
  • Preparation method for biological agent for preventing and controlling human papilloma virus infection
  • Preparation method for biological agent for preventing and controlling human papilloma virus infection

Examples

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experiment example 1

[0022] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:

[0023] (1) Separation and purification of β-lactoglobulin-based stable proteins from milk products;

[0024] (2) Use active acid anhydride to acid anhydride the separated and purified protein, and then purify the active protein again after dialysis;

[0025] 3-Hydroxy-phthalic anhydride (HP) was first dissolved in dimethylsulfoxide DMSO to a saturated concentration, then β-lactoglobulin (β-LG) was dissolved in pH 8.5, 0.1M sodium phosphate In the solution, make the final protein concentration 20mg / mL, then add the above-mentioned HP into the protein solution, oscillate and mix well, place it for 1 hour at a temperature of 25°C, dialyze with pH7.4 PBS, and then use 0.45uM microporous Sterilize by filter membrane, store at 4°C, and then make a gel according to the existing method.

experiment example 2

[0027] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:

[0028] First, dissolve HP in dimethyl sulfoxide DMSO until the concentration reaches saturation, then dissolve chicken serum albumin OVA in pH 8.5, 0.1M sodium phosphate solution, so that the final protein concentration is 20 mg / mL, and then dissolve the above HP solution Divide into five equal parts, add to the protein solution every 12 minutes, vortex and mix well, adjust the pH to 8.5 with 1M NaOH, and the final concentration of acid anhydride in the reaction system is 60mM. At a temperature of 25°C, place it for 1 hour, dialyze the pH7.4 phosphate buffer solution PBS at 4°C overnight, then filter and sterilize with a 0.45uM microporous membrane, store at 4°C, and then make a paste according to the existing method cream.

experiment example 3

[0030] A preparation method for preventing and controlling a biological agent of human papillomavirus infection, comprising the following steps:

[0031] Firstly, HP was dissolved in dimethyl sulfoxide DMSO, the concentration reached saturation (1.19M), then rabbit serum protein RSA was dissolved in pH8.5, 0.1M sodium phosphate solution, so that the final protein concentration was 20mg / mL, and then The above HP solution was divided into five equal parts, added to the protein solution every 12 minutes, oscillated and mixed, 1M NaOH was used to adjust the pH to 8.5, and the final concentration of acid anhydride in the reaction system was 60 mM. At a temperature of 25°C, place it for 1 hour, dialyze the pH7.4 phosphate buffer solution PBS at 4 degrees overnight, then filter and sterilize it with a 0.45uM microporous membrane, store it at 4°C, and then make a spray according to the existing method agent.

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Abstract

The invention discloses a preparation method for a biological agent for preventing and controlling human papilloma virus infection. The preparation method includes following steps: dissolving 3-hydroxy-phthalic anhydride HP into dimethyl sulfoxide DMSO to obtain saturated HP solution; dissolving beta-lactoglobulin beta-LG into 0.1M of sodium phosphate solution with pH (potential of hydrogen) of 8.5 to obtain protein solution with protein final concentration of 20mg / mL; and equally dividing the HP solution into five parts, adding the HP solution into the protein solution at every 12 minutes, shaking and mixing, adjusting pH of IMNaOH to be 8.5, leading the final concentration of anhydride in a reaction system to be 60mM, standing for 1 hour at the temperature of 25 DEG C, dialyzing by PBS (phosphate buffer solution) with pH of 7.4, performing filtration sterilization by a 0.45uM microfiltration membrane, and storing at the temperature of 4 DEG C so as to obtain a finished product. The biological agent has the advantages of capabilities of stopping HPV (human papilloma virus) from invading cells and stopping virus infection from spreading, safety, stability, low cost and the like.

Description

Technical field [0001] The invention involves a new type of antiviral biological agent in the field of pharmaceutical technology, which specifically involves a preparation method that involves a preparation and controlling human papilloma virus infection. Background technique [0002] Human Pparanoma Virus (HPV) belongs to Papillomaviridae, which is a type of epithelial dual -chain DNA virus.So far, the HPV separated in the human skin and mucosal tissue can be divided into more than 100 types according to the different DNA sequences.It can be divided into "low -risk" type and "high -risk" type according to its carcinogenicity.The main cause of tissue malignant tumors, especially cervical cancer. [0003] Cervical cancer has become the second largest malignant tumor that endangers women's health. Studies have found that more than 99.7%of the pathological tissue of cervical cancer can detect HPV DNA. ThereforeCancer caused by HPV infection.At present, the disease is about 500,000 n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K38/38C07K1/107A61P31/20
Inventor 杨霞姜世勃陆路
Owner SHANXI JINBO BIO PHARMA CO LTD
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